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Ramyashri S, Aparna Rao, PRITY SAHAY; Iridial vasculature and pericyte morphology in different types of glaucoma.. Invest. Ophthalmol. Vis. Sci. 2017;58(8):2098.
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© ARVO (1962-2015); The Authors (2016-present)
To study iridial vasculature and pericyte morphology of iris vessels in different types of glaucoma
Iris samples were obtained from patients who underwent standard trabeculectomy after informed consent .Tissues were fixed in 10% formalin and then cut at 4 µm size thin sections. The sections were collected in poly L lysine coated slides and stained with routine haematoxylin and eosin,examined under light microscope with 10x, 20x,40x and 100x magnification.Iris morphology was compared in glaucoma cases and control iris tissue obtained from enucleated eyes.
Iris specimens were obtained from patients with POAG(Primary open angle glaucoma), PACG(Primary angle closure glaucoma) and PCG(Primary congenital glaucoma) (n=1 each) who underwent trabeculectomy which were compared with iris morphology obtained from enucleated eye.Iris from glaucoma cases (POAG and PACG) appeared to have significantly narrowed inner luminal calibre with thickened tunica media as compared to control. Interestingly, the endothelial cells were very sparse and significantly reduced with flattened pericytes in iris of glaucoma cases which was seen in POAG, PACG and PCG compared to controls.
A definite reduction in iridial vessel calibre with reduced pericytes are novel findings which suggest structural damage to pericytes and basement membrane barrier functions in different glaucoma types.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
Histopathology section of Iris in control under 20x magnification showing thin vessel wall with plump endothelium and pericytes lining vessel wall.(Inset black arrows).
Histopathology section of Iris in PCG under 20x magnification showing thin vessel wall with flattened endothelial cells and sparse flat pericytes.(Inset white arrows).
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