June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Real time, non invasive, assessment of transepithelial delivery of riboflavin in the human cornea
Author Affiliations & Notes
  • Giuseppe Lombardo
    National Research Council, Messina (Me), Italy
    Vision Engineering Italy srl, Rome, Italy
  • Michael Brownell
    Independent, San Diego, California, United States
  • Valentina Villari
    National Research Council, Messina (Me), Italy
  • Norberto Liborio Micali
    National Research Council, Messina (Me), Italy
  • Edward E Manche
    Department of Ophthalmology, Stanford University School of Medicine , Stanford, California, United States
  • Anthony Abbate
    Independent, San Diego, California, United States
  • Marco Lombardo
    Vision Engineering Italy srl, Rome, Italy
  • Footnotes
    Commercial Relationships   Giuseppe Lombardo, Vision Engineering Italy srl (P); Michael Brownell, Seros Medical LCC (E); Valentina Villari, Vision Engineering Italy srl (P); Norberto Liborio Micali, Vision Engineering Italy srl (P); Edward Manche, Seros Medical LCC (P); Anthony Abbate, Seros Medical LCC (P); Marco Lombardo, Vision Engineering Italy srl (P)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 3518. doi:
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      Giuseppe Lombardo, Michael Brownell, Valentina Villari, Norberto Liborio Micali, Edward E Manche, Anthony Abbate, Marco Lombardo; Real time, non invasive, assessment of transepithelial delivery of riboflavin in the human cornea. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3518.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Current protocols do not quantify riboflavin concentration or cross-linking and typically require removal of the corneal epithelium. This study measures the concentration of stromal riboflavin in vacuum-assisted transepithelial corneal cross-linking using a novel UV-A theranostics device.

Methods : Nine human donor corneoscleral tissues with intact epithelium underwent transepithelial corneal cross-linking using a vacuum-assisted device for delivering dextran-free, hypotonic, 0.1% riboflavin with enhancers, and irradiating the cornea at 12 mW/cm2 for 9 minutes. The drug delivery device was composed of a small chamber with a perforated contoured surface in contact with the epithelium and a vacuum pump to control chamber air pressure. Seven additional control human donor corneoscleral tissues were de-epithelialized and soaked with 20% dextran-enriched 0.1% riboflavin solution for 30 minutes and then irradiated with 10 mW/cm2 for 9 minutes and used as controls. In both cases, the UV-A device (Chromo4Vis) used was based on theranostics technology, which provided real time measurement of the intrastromal concentration of riboflavin during treatment and assisted the user to best tailor corneal cross-linking to each corneal tissue.

Results : Successful transepithelial riboflavin deposition with the vacuum-mediated device was achieved in all corneas with an average stromal concentration of 0.009%±0.003% and a maximum of 0.017%. After transepithelial UV-A irradiation, the intrastromal concentration of riboflavin decreased to 0.002%±0.0006%. In the epithelium-off control tissues, the average riboflavin concentration was 0.016% ± 0.003% after soaking; it decreased to 0.004% ± 0.002% after UV-A irradiation of the corneal stroma. In all cases, the stromal riboflavin concentration decreased non-linearly during UV-A irradiation of the cornea.

Conclusions : The novel UV-A theranostics device successfully quantified stromal riboflavin concentration and monitored the corneal cross-linking process in real time. The vacuum-assisted device demonstrated transepithelial riboflavin deposition at concentration levels comparable to the control tissues with epithelium removed.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

 

Time dependent concentration values of intrastromal riboflavin collected in two representative cases during UV-A irradiation by the novel Chromo4Vis theranostics technology

Time dependent concentration values of intrastromal riboflavin collected in two representative cases during UV-A irradiation by the novel Chromo4Vis theranostics technology

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