June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Effects of proteoglycan decorin on lens epithelial cells
Author Affiliations & Notes
  • Shinsuke Shibata
    Ophthalmology, Kanazawa Medical University, Ishikawa, Japan
  • Naoko Shibata
    Ophthalmology, Kanazawa Medical University, Ishikawa, Japan
  • Teppei Shibata
    Ophthalmology, Kanazawa Medical University, Ishikawa, Japan
  • Naoki Tanimura
    Ophthalmology, Kanazawa Medical University, Ishikawa, Japan
  • Hiroshi Sasaki
    Ophthalmology, Kanazawa Medical University, Ishikawa, Japan
  • Eri Kubo
    Ophthalmology, Kanazawa Medical University, Ishikawa, Japan
  • Footnotes
    Commercial Relationships   Shinsuke Shibata, None; Naoko Shibata, None; Teppei Shibata, None; Naoki Tanimura, None; Hiroshi Sasaki, None; Eri Kubo, None
  • Footnotes
    Support  Grant-in-Aid for Young Scientists (B) 16K20336
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 1703. doi:
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    • Get Citation

      Shinsuke Shibata, Naoko Shibata, Teppei Shibata, Naoki Tanimura, Hiroshi Sasaki, Eri Kubo; Effects of proteoglycan decorin on lens epithelial cells. Invest. Ophthalmol. Vis. Sci. 2017;58(8):1703.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Involvement of cytokines including TGFβ2 and FGF2 has been reported in the onset of postoperative posterior capsule opacity (PCO). Previously, we showed that expression level of proteoglycan decorin (DCN) was highly up-regulated in lens epithelial cells (LEC) in a rat posterior capsule opacity (PCO) model. In this study, we analyzed DCN expression in cultured LEC treated with TGFβ2 and/or FGFβ2, and the effects of DCN on cell proliferation.

Methods : Primary cultured mouse LECs (MLEC) and human LEC (HLEC:SRA01/04) were growing in DMEM containing 2% fetal bovine serum were treated with 0 to 10ng/ml of FGF2 and 0 to 10ng/ml TGFβ2. Expressions of DCN and tropomyosin (Tpm) were measured using real time RT-RCR. Cell viability assay was conducted by MTS assay using MLEC and HLEC. Data were reported as means ± SDs and analyzed by 1-way ANOVA, followed by a t test when appropriate, with p< 0.05 deemed significant.

Results : After addition of FGF2, expression of DCN mRNA levels was increased in HLEC (p<0.05) and MLEC (Fig. 1; p<0.01). In contrast, expression of Tpm1 mRNA was decreased after addition of FGF2 in HLEC (p<0.05). Addition of TGFβ2 suppressed the expression of DCN mRNA (p<0.01). However, addition of DCN to MLEC and HLEC did not affect cell viability.

Conclusions : Upregulation of DCN in LEC detected in rat PCO model may be induced by FGF2. Because DCN was not induced by addition of TGF, DCN may not play an important role in EMT of LEC. Further investigation is needed to reveal the relationship between DCN and progression of PCO in LEC.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

 

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