June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Bactec™ blood culture bottles allied to MALDI-TOF mass spectrometry: Rapid etiologic diagnosis of bacterial endophthalmitis
Author Affiliations & Notes
  • Tatiana Tanaka
    Ophthalmology, University of São Paulo, São Paulo, Brazil
  • Luiza Manhezi de Freitas Oliveira
    Ophthalmology, University of São Paulo, São Paulo, Brazil
  • Bruno Fortaleza de Aquino Ferreira
    Ophthalmology, University of São Paulo, São Paulo, Brazil
  • Juliana Mika Kato
    Ophthalmology, University of São Paulo, São Paulo, Brazil
  • Flavia Rossi
    Central Laboratory Division, University of São Paulo, São Paulo, Brazil
  • Karoline de Lemes Giuntini Correa
    Central Laboratory Division, University of São Paulo, São Paulo, Brazil
  • Sergio Luis Gianotti Pimentel
    Ophthalmology, University of São Paulo, São Paulo, Brazil
  • Joyce H Yamamoto
    Ophthalmology, University of São Paulo, São Paulo, Brazil
  • Joao Nobrega de Almeida Junior
    Central Laboratory Division, University of São Paulo, São Paulo, Brazil
  • Footnotes
    Commercial Relationships   Tatiana Tanaka, None; Luiza Oliveira, None; Bruno Ferreira, None; Juliana Kato, None; Flavia Rossi, None; Karoline Correa, None; Sergio Pimentel, None; Joyce Yamamoto, None; Joao Junior, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 3883. doi:
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      Tatiana Tanaka, Luiza Manhezi de Freitas Oliveira, Bruno Fortaleza de Aquino Ferreira, Juliana Mika Kato, Flavia Rossi, Karoline de Lemes Giuntini Correa, Sergio Luis Gianotti Pimentel, Joyce H Yamamoto, Joao Nobrega de Almeida Junior; Bactec™ blood culture bottles allied to MALDI-TOF mass spectrometry: Rapid etiologic diagnosis of bacterial endophthalmitis. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3883.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To evaluate matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) for direct identification (ID) of the etiologic agents of endophthalmitis from vitreous humor-inoculated blood culture bottles (BCBs).

Methods : A prospective study was conducted in a tertiary hospital in São Paulo, Brazil. From October 2015 to November 2016, patients with clinical suspicion of endophthalmitis had vitreous humor samples inoculated in blood culture bottles (BCBs). Positive BCBs were initially seeded in culture plates according to standard procedures. After standard procedure, aliquots from positive BCBs were immediately submitted to a simple in-house protein extraction protocol for direct ID by MALDI-TOF MS. The microorganisms’ ID and turnaround time (TAT) by standard method and direct MALDI-TOF analysis were compared.

Results : Forty-one samples of vitreous humor from 31 patients were analyzed. In 90.2% of cases, direct MALDI-TOF MS analysis from positive BCBs successfully identified the pathogen. The median TAT for pathogen ID by standard subculture and direct MALDI-TOF MS analysis was 70.2 h (32.5 h-212.9 h) and 14.7 h (2.4 h - 94.0 h), respectively (p <0.0001). Box-and-Whiskers plot of the TAT for pathogen identification according to methodology is represented below (Direct: TAT for direct pathogen identification from vitreous humor-inoculated blood culture bottles by MALDI-TOF mass spectrometry. Standard: TAT for pathogen identification after subculture of from blood culture bottles).

Conclusions : Direct pathogen ID of vitreous humor-inoculated BCBs by MALDI-TOF MS is an accurate and useful technique for rapid ID of the causative agents of endophthalmitis.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

 

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