June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
In vivo assessments of retinal structure and function, and anterograde optic nerve transport after whole eye transplantation
Author Affiliations & Notes
  • Chiaki Komatsu
    Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
  • Yolandi van der Merwe
    Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
    Neuroimaging Laboratory, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
  • Lin He
    Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
  • Maxine R. Miller
    Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
  • Katie A. Lucy
    Department of Ophthalmology, New York University , New York, New York, United States
  • Michael Steketee
    Department of Ophthalmology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
  • Gadi Wollstein
    Department of Ophthalmology, New York University , New York, New York, United States
  • Joel S Schuman
    Department of Ophthalmology, New York University , New York, New York, United States
  • Kevin C Chan
    Neuroimaging Laboratory, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
    Department of Ophthalmology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
  • Kia M. Washington
    Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
    Veterans Administration Pittsburgh Healthcare System, Pittsburgh, Pennsylvania, United States
  • Footnotes
    Commercial Relationships   Chiaki Komatsu, None; Yolandi van der Merwe, None; Lin He, None; Maxine Miller, None; Katie Lucy, None; Michael Steketee, None; Gadi Wollstein, None; Joel Schuman, Zeiss (P); Kevin Chan, None; Kia Washington, None
  • Footnotes
    Support  Office of the Assistant Secretary of Defense for Health Affairs under Award No. W81XWH-14-1-0421, VA Pittsburgh Healthcare Administration, National Institutes of Health P30-EY008098 (Bethesda, Maryland); Eye and Ear Foundation (Pittsburgh, Pennsylvania); and Research to Prevent Blindness (New York, New York).
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 4158. doi:
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      Chiaki Komatsu, Yolandi van der Merwe, Lin He, Maxine R. Miller, Katie A. Lucy, Michael Steketee, Gadi Wollstein, Joel S Schuman, Kevin C Chan, Kia M. Washington; In vivo assessments of retinal structure and function, and anterograde optic nerve transport after whole eye transplantation. Invest. Ophthalmol. Vis. Sci. 2017;58(8):4158.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We aim to assess retinal structural integrity, anterograde transport of the visual system and retinal function with optical coherence tomography (OCT), manganese (Mn)-enhanced MRI (MEMRI) and electroretinography (ERG) in our recently established whole eye transplantation (WET) rat model.

Methods : Syngeneic orthotopic WET were performed to the right eyes of 9 Lewis rats (Fig.1-A) The donor flaps consisted of tissue anterior to the optic chiasm, a section of temporal bone and skin of the eyelid and external ear. A similar region of tissue was removed in the recipient with the exception of the optic nerve (ON), which was cut at the base of the globe. ON coaptations, anastomoses of carotid arteries and external jugular veins were performed between donors and recipients. OCT was performed at week 1 after WET (n=7). MEMRI was performed at week 3 to evaluate anterograde Mn transport along the visual pathway after binocular intravitreal Mn injections. ERG was performed at week 7 in 2 separate animals to evaluate retinal function.

Results : OCT revealed visualization of retinal layers in 5 of the 7 transplanted eyes examined (Fig.1-B), whereas the other 2 eyes had corneal opacities that prevented visualization. MEMRI showed that after intravitreal Mn injections to both eyes, Mn transported anterogradely from the left, untreated eye to the right visual brain centers (Fig.2). Mn enhancement was also observed in the right, transplanted donor eye and intraorbital ON when compared to the eye and visual system prior to Mn injections. No apparent difference in Mn enhancement was observed between left naïve and right transplanted intraorbital ON. No apparent Mn enhancement was observed in the right recipient prechiasmatic ON, left lateral geniculate nucleus or left superior colliculus. In ERG, the transplanted eyes showed a-and b-waves in response to light stimulation.

Conclusions : OCT imaging revealed the existence of retinal layers after WET. MEMRI data suggested the presence of anterograde Mn transport from the donor eye to the optic nerve up to the site of transection. ERG data suggested some responses to light stimuli in the photoreceptors of transplanted eyes. The ocular viability seen after WET allows the potential for future therapeutic strategies for neuroprotection, ON regeneration and vision restoration.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

 

 

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