June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Corneal findings after death: a preliminary OCT study on an animal model
Author Affiliations & Notes
  • Matteo Nioi
    Department of Public Health, Clinical and Molecular Medicine -Forensic Scinece Unit-, University of Cagliari, CA (Cagliari), Italy
  • Pietro Emanuele Napoli
    Department of Surgical Sciences, Eye Clinic, University of Cagliari, Cagliari, Sardinia, Italy
  • Claudio Iovino
    Department of Surgical Sciences, Eye Clinic, University of Cagliari, Cagliari, Sardinia, Italy
  • Francesco Paribello
    Department of Surgical Sciences, Eye Clinic, University of Cagliari, Cagliari, Sardinia, Italy
  • Maurizio Fossarello
    Department of Surgical Sciences, Eye Clinic, University of Cagliari, Cagliari, Sardinia, Italy
  • Ernesto d'Aloja
    Department of Public Health, Clinical and Molecular Medicine -Forensic Scinece Unit-, University of Cagliari, CA (Cagliari), Italy
  • Footnotes
    Commercial Relationships   Matteo Nioi, None; Pietro Napoli, None; Claudio Iovino, None; Francesco Paribello, None; Maurizio Fossarello, None; Ernesto d'Aloja, None
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 4873. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Matteo Nioi, Pietro Emanuele Napoli, Claudio Iovino, Francesco Paribello, Maurizio Fossarello, Ernesto d'Aloja; Corneal findings after death: a preliminary OCT study on an animal model. Invest. Ophthalmol. Vis. Sci. 2017;58(8):4873.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : The aim of this study was to describe by means of a portable OCT the postmortem morphological changes of the central cornea in an animal model (Ovis aries).

Methods : The design involved the analysis of 30 eyes (15 heads) of female, adult sheep (> 2 years) sacrificed at a local slaughterhouse. The eyes, after decapitation, were examined in situ, without enucleation. Ocular globes were stored at known temperature (within a range of 12–22 °C) and humidity (within a range of 50–60%). The instrumental analysis was executed using a portable spectral-domain OCT (SD-OCT) system (iVue SD-OCT, Optovue Inc, Fremont, CA) calibrated to the corneal mode. OCT imaging was performed at the following time-points since death: immediately (within 15minutes), at the 30th minute, at the 1st, 2nd, 3rd, 4th, 5th, 6th, 7th, 8th, 9th, 10th, 11th, 12th, 15th, 18th, 21st, 24th, 30th , 36th ,42nd, 48thhour, and later (< 72nd). Pachymetric map, morphological and ultrastructural analysis (epithelium, stroma, and endothelium) were performed for each time-point.

Results : After initial thinning and epithelial hyper-reflectivity, stromal thickness increased from the 2nd up to the 6th hour. Subsequently, a new trend of thinning was observed in association with a demarcation line between anterior and posterior stroma. At the same time, in 33% of samples, epithelium disappeared and endothelium was characterized by an increase of reflectivity. After the 12th hour, a recurrence of corneal swelling was detected in association with the delamination of stromal tissue. Since the 24th hour, epithelium disappeared in 50% of cases and anterior chamber depth progressively decreased. At the 48th hour, corneal tissue showed the onset of putrefaction processes: the appearance of hyper-reflective dots in anterior chamber, irido-corneal contact, and the massive vacuolization of the posterior stroma until the total delamination.

Conclusions : The portable OCT system is a useful approach for in situ, postmortem corneal examination, and may be potentially applied for the selection of donor cornea in transplantology and the determination of post-mortem intervals in forensic medicine.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

 

OCT evidences during the putrefaction process of the cornea throughout the late intervals: the irido-corneal contact, the massive vacuolization of the posterior stroma until the delamination and the appearance of hyper-reflective dots in anterior chamber.

OCT evidences during the putrefaction process of the cornea throughout the late intervals: the irido-corneal contact, the massive vacuolization of the posterior stroma until the delamination and the appearance of hyper-reflective dots in anterior chamber.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×