June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Dark adaptation in Duchenne muscular dystrophy with a short protocol
Author Affiliations & Notes
  • Balazs VINCE Nagy
    Department of Mechatronics, Optics and Engineering Informatics, Budapest University of Technology and Economics, Budapest, Hungary
    Institute of Psychology, University of Sao Paulo, SAO PAULO, SP, Brazil
  • Zahra Ashman
    University at Buffalo, Buffalo, New York, United States
  • Mirella Telles Salgueiro Barboni
    Institute of Psychology, University of Sao Paulo, SAO PAULO, SP, Brazil
  • Leonardo Padua
    Institute of Psychology, University of Sao Paulo, SAO PAULO, SP, Brazil
  • Kallene Summer Moreira Vidal
    Institute of Psychology, University of Sao Paulo, SAO PAULO, SP, Brazil
  • Dora Fix Ventura
    Institute of Psychology, University of Sao Paulo, SAO PAULO, SP, Brazil
  • Footnotes
    Commercial Relationships   Balazs Nagy, None; Zahra Ashman, None; Mirella Barboni, None; Leonardo Padua, None; Kallene Vidal, None; Dora Ventura, None
  • Footnotes
    Support  FAPESP (Projeto Temático 2014/26818-2; Projeto Regular 2016/04538-3; Pesquisador Visitante 2014/06457-5); CNPq (Auxílio individual 470785/2014-4); CAPES (Programa Pró-Amazônia 3263/2013); NIH T37MD001378-16.
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 5408. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Balazs VINCE Nagy, Zahra Ashman, Mirella Telles Salgueiro Barboni, Leonardo Padua, Kallene Summer Moreira Vidal, Dora Fix Ventura; Dark adaptation in Duchenne muscular dystrophy with a short protocol. Invest. Ophthalmol. Vis. Sci. 2017;58(8):5408.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To investigate if Duchenne Muscular Dystrophy affects dark adaptation for the rods and cones of the eye with a new short psychophysical protocol.

Methods : In this pilot study two Duchenne Muscular Dystrophy (DMD) patients and eleven healthy volunteers were tested monocularly. The tests were conducted using the Roland Consult Darkadaptometer system. Instead of the standard 45-minute protocol we have developed a 8+6 minute test to be able to test DMD patients avoiding muscular fatigue. The protocol begins with one minute of bleaching with white light at 3000 cd/m2. The first part of the test lasts 8 minutes to verify the separation of rod and cone adaptation levels (rod-cone break). For this interleaved red and green stimulation flashes of dynamically changing luminance (depending on patient response with a button control) are shown inside a Ganzfeld stimulator at 20 degrees temporal eccentricity. After a 5-minute interval with eyes closed, a 6-minute test session is performed with green stimuli only to test for rod adaptation.

Results : The test was evaluated with the control group having an average rod-cone break luminance of -1.82±0.087 log cd/m2 at 148±71 seconds and an average stable rod adaptation luminance of -4.04±0.188 log cd/m2. Relative to controls, the time and luminance for the rod-cone break of the first DMD patient (with genetic mutation upstream exon 30) falls within the normal range (Fig 1), just as the luminance of the stabilized rod adaptation level (Fig 2). The second DMD patient (with genetic mutation downstream exon 30) did not reach the rod/cone break within the 8 minutes of the first test phase while his stable red adaptation luminance level stayed within the control group’s range (-1,93 log cd/m2). Between the two DMD patients there was a large difference in the rod adaptation luminance (-4 log cd/m2 vs -2.7 log cd/m2).

Conclusions : The newly developed short dark adaptation protocol used in this study performed well as verified with the control group since it demonstrated low variability among subjects. The DMD patient lacking only Dp427 genetic mutation had results that indicate a conservation of rod and cone adaptation in DMD while the lack of smaller DMD gene products, such as Dp260 (mainly expressed by the rods) of the second patient indicate impairment of rod vision affecting dark adaptation as well.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

 

 

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×