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Mohamed T El-Haddad, Karen M Joos, Shriji Patel, Yuankai Tao; Wide-field multimodal ophthalmic imaging using scanning laser ophthalmoscopy and optical coherence tomography at 400 kHz. Invest. Ophthalmol. Vis. Sci. 2017;58(8):5446.
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© ARVO (1962-2015); The Authors (2016-present)
Scanning laser ophthalmoscopy (SLO) and optical coherence tomography (OCT) enable in vivo imaging of ophthalmic structures. Multimodality SLO-OCT systems provide complementary en face and cross-sectional information for aiming, bulk motion registration, and multi-field mosaicking. We previously demonstrated a swept-source spectrally-encoded SLO and OCT (SS-SESLO-OCT) system using single-mode illumination and multimode collection through a double-clad fiber coupler to improve collection efficiency and reduce speckle contrast. Here, we present a novel SS-SESLO-OCT system for wide-field imaging at 400 kHz line-rate.
SESLO and OCT illumination and optical triggering and clocking used a shared 1060 nm Axsun swept-source optically buffered to 400 kHz. SESLO and OCT images were acquired simultaneously on a dual-channel digitizer with a combined throughput of 2.4 GS/s using shared imaging optics and digitization, triggering, and clocking electronics (Fig. 1(a), (b)).
In vivo ophthalmic imaging was performed in a healthy volunteer under an IRB-approved protocol. Corneal (Fig. 1(c)-(e)) and widefield retinal (Fig. 1(f)-(h)) images were sampled at 2560 x 2000 pix. (spectral x lateral) with 1400 frames-per-volume at 200 frames-per-second. A full en face SESLO frame was acquired simultaneously with each OCT cross-section. Multimode collection provided >3x increase in collection efficiency and >3.5x reduction in speckle contrast.
SS-SESLO-OCT enables high-speed multimodal widefield imaging of ocular structures. Simultaneous en face and cross-sectional imaging provides complementary information that may be used for image aiming, retinal tracking, and multi-volumetric registration, averaging, and mosaicking.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
Fig.1. In vivo corneal and retinal imaging using SS-SESLO-OCT. (a) SS-SESLO-OCT illumination and detection engine and (b) imaging relay. (c), (f) Single-frame and (d), (g) 5-frame averaged SESLO images. (e), (h) En face and cross-sectional OCT images. DCF, double-clad fiber; FBG, fiber Bragg grating; f, collimating, objective, ophthalmic, relay, and scan lenses; Gx,y, galvanometer scanners; M, mirror; MMF, multimode fiber; PC, polarization controller; BPD, balanced photodiode; PM, prism mirror; VPHG, grating; WP, wedge prism.
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