June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Effect of 0.1% Fluorescein dye on Riboflavin Photodynamic Antimicrobial Therapy for inhibition of Methicillin-Resistant Staphylococcus aureus.
Author Affiliations & Notes
  • Nidhi Relhan
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Heather Ann Durkee
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Mariela C Aguilar
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Alejandro Arboleda
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Nicholas Nolan
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Anna Martinez
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Guillermo Amescua
    Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Harry W Flynn
    Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Darlene Miller
    Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, Florida, United States
    Ocular Microbiology Laboratory, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
  • Jean-Marie A Parel
    Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, United States
    CHU Sart-Tillman, Department of Ophthalmology, University of Liege, Liege, Belgium
  • Footnotes
    Commercial Relationships   Nidhi Relhan, None; Heather Durkee, None; Mariela Aguilar, None; Alejandro Arboleda, None; Nicholas Nolan, None; Anna Martinez, None; Guillermo Amescua, None; Harry Flynn, None; Darlene Miller, None; Jean-Marie Parel, None
  • Footnotes
    Support  Supported in part by the Janet K and Edward D. Robson Foundation, Florida Lions Eye Bank, Drs. KR Olsen and ME Hildebrandt, Drs. Raksha Urs and Aaron Furtado, Center Grant P30EY14801, Research to Prevent Blindness, and the Henri and Flore Lesieur Foundation
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 3895. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Nidhi Relhan, Heather Ann Durkee, Mariela C Aguilar, Alejandro Arboleda, Nicholas Nolan, Anna Martinez, Guillermo Amescua, Harry W Flynn, Darlene Miller, Jean-Marie A Parel; Effect of 0.1% Fluorescein dye on Riboflavin Photodynamic Antimicrobial Therapy for inhibition of Methicillin-Resistant Staphylococcus aureus. . Invest. Ophthalmol. Vis. Sci. 2017;58(8):3895.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Controversy exists regarding the use of fluorescein prior to riboflavin mediated photodynamic antimicrobial therapy (PDAT). Some researchers report that fluorescein used to stain corneal epithelium, competes with riboflavin for the absorption of UV-A and thus reduces the antimicrobial effect of the PDAT procedure. The hypothesis that fluorescein dye has an inhibitory effect on the riboflavin mediated PDAT was evaluated in the current study.

Methods : MRSA strains were isolated from the corneal scraping of patients with confirmed bacterial keratitis. The MRSA isolates were mixed with appropriate photosensitizing solutions (0.1% riboflavin or 0.1% fluorescein or 0.1% riboflavin with 0.1% fluorescein) and plated on blood agar plates which were then exposed to PDAT (UV-A radiation exposure for 15-minutes). The custom-built UV-A light source 365 nm LED provided total energy of 5.4J/cm2 (Dresden protocol). After the light exposure, all plates were placed in an incubator at 30°C and were photographed after 48 hours.

Results : Four strains of MRSA (2 healthcare-associated and 2 community-associated) were used for the in-vitro experiments. The control agar plates (Fig 1A) showed growth of MRSA isolates indicating the presence of viable organisms. Among the agar plates exposed to UV-A light: i) 0.1% riboflavin (Fig 1B), ii) 0.1% fluorescein (Fig 1C) and, iii) 0.1% fluorescein + 0.1% riboflavin (Fig 1D), there was no difference in the growth pattern of MRSA isolates. MRSA isolates grew all over the agar plates irrespective of the use of 0.1% fluorescein dye.

Conclusions : The current study showed that the riboflavin-mediated PDAT has no beneficial effect for the inhibition of MRSA strains. Also, use of 0.1% fluorescein dye did not affect the efficacy of riboflavin-mediated PDAT.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

 

Figure 1. Agar culture plates with 4 MRSA strains. Control group (A) and groups with 15 minutes of UV-A light exposure (B), (C) & (D) show growth of MRSA all over the plates irrespective of the use of fluorescein or riboflavin. The area within the small blue circles indicate the area of light exposure during PDAT.
(Abbreviations - PDAT- photodynamic antimicrobial therapy, MRSA- Methicillin-resistant Staphylococcus aureus, HA- healthcare-associated MRSA, CA-community-associated MRSA)

Figure 1. Agar culture plates with 4 MRSA strains. Control group (A) and groups with 15 minutes of UV-A light exposure (B), (C) & (D) show growth of MRSA all over the plates irrespective of the use of fluorescein or riboflavin. The area within the small blue circles indicate the area of light exposure during PDAT.
(Abbreviations - PDAT- photodynamic antimicrobial therapy, MRSA- Methicillin-resistant Staphylococcus aureus, HA- healthcare-associated MRSA, CA-community-associated MRSA)

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×