June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
The Role of the Oxytocin and Secretin Receptors in Modulating Inflammation in Ocular Surface Tissues
Author Affiliations & Notes
  • Aliaa Abdelhakim
    Harkness Eye Institute, Columbia University Medical Center, New York, New York, United States
  • Muhammad Anwar
    Department of Psychiatry, Columbia University Medical Center, New York, New York, United States
  • Lauren Rosko
    Department of Psychiatry, Columbia University Medical Center, New York, New York, United States
  • James Todaro
    Harkness Eye Institute, Columbia University Medical Center, New York, New York, United States
  • Takayuki Nagasaki
    Harkness Eye Institute, Columbia University Medical Center, New York, New York, United States
  • Robert Ludwig
    Department of Pediatrics, Columbia University Medical Center, New York, New York, United States
  • Martha Welch
    Department of Psychiatry, Columbia University Medical Center, New York, New York, United States
    Department of Pediatrics, Columbia University Medical Center, New York, New York, United States
  • Bryan J Winn
    Harkness Eye Institute, Columbia University Medical Center, New York, New York, United States
  • Footnotes
    Commercial Relationships   Aliaa Abdelhakim, None; Muhammad Anwar, None; Lauren Rosko, None; James Todaro, None; Takayuki Nagasaki, None; Robert Ludwig, None; Martha Welch, None; Bryan Winn, None
  • Footnotes
    Support  Research to Prevent Blindness Departmental Grant
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 3948. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Aliaa Abdelhakim, Muhammad Anwar, Lauren Rosko, James Todaro, Takayuki Nagasaki, Robert Ludwig, Martha Welch, Bryan J Winn; The Role of the Oxytocin and Secretin Receptors in Modulating Inflammation in Ocular Surface Tissues. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3948.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Inflammatory diseases of the ocular surface occur with high prevalence. Previous studies have shown that co-administration of oxytocin and secretin reduced gut inflammation elicited by experimental colitis in rodents. Here, we investigate the presence of the oxytocin and secretin receptors (OXTR and SCTR) on the corneal and conjunctival surfaces and their role as possible modulators of ocular surface inflammation.

Methods : We used immunofluorescence to localize OXTR and SCTR in paraffinated sections of rodent and human eyes, and in the SV40-HCEC human cornea cell line. To test the effects of OXTR and SCTR in vitro, we created a cell culture model of ocular surface inflammation by adding the inflammatory cytokine TNF-α to SV40-HCEC cells and measuring the resultant levels of the inflammatory marker ICAM-1 by Western blot. The program ImageJ was used to quantify signals.

Results : OXTR was expressed in the superficial layer of human corneal and conjunctival epithelia (Fig 1a); this pattern was also observed in rat eyes. OXTR was also expressed in SV40-HCEC cells. SCTR was detected in the human and rat cornea (Fig 1b) and in SV40-HCEC cells. Expression of both receptors was confirmed in SV40-HCEC lysate by Western blotting. To test the anti-inflammatory effects of oxytocin and secretin in vitro, we measured ICAM-1 expression levels in SV40-HCEC cells after individual or combined oxytocin and secretin treatment. Levels of ICAM-1 were compared with TNF-α alone, TNF-α plus oxytocin, TNF-α plus secretin, or TNF-α with both hormones added (Fig 1c). ICAM-1 levels 3 hours after addition of oxytocin were ~40% of that measured with TNF-α treatment alone. Secretin similarly decreased ICAM-1 levels to ~40% at the same time point. The combination of the two drugs resulted in no further reduction in ICAM-1 levels. Effects of the hormones were transient with the maximal effect at 3-4 hours after hormone addition.

Conclusions : OXTR and SCTR are present on humans and rodent ocular surface tissues. Addition of oxytocin and/or secretin appears to decrease inflammation in cell culture as measured by ICAM-1 levels. These data suggest an anti-inflammatory role for these receptors on the ocular surface and possible use as therapeutic targets in ocular surface disease.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

 

Figure 1. a: OXTR expression, human cornea. b: SCTR expression, human cornea. c: Effect of oxytocin and secretin on ICAM-1 levels, SV40-HCEC cells.

Figure 1. a: OXTR expression, human cornea. b: SCTR expression, human cornea. c: Effect of oxytocin and secretin on ICAM-1 levels, SV40-HCEC cells.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×