June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Anti-inflammation effects of Zidovudine in human corneal epithelial cells
Author Affiliations & Notes
  • hui liu
    Ophthalmology, Loyola University Chicago, Maywood, Illinois, United States
    Tianjin Medical University Eye Hospital, Tianjin, China
  • Frank Gambino
    Microbiolology & Immunology, Loyola University Chicago, Maywood, Illinois, United States
  • Taylor Keller
    Microbiolology & Immunology, Loyola University Chicago, Maywood, Illinois, United States
  • Yougang Zhai
    Microbiolology & Immunology, Loyola University Chicago, Maywood, Illinois, United States
  • Liang Qiao
    Microbiolology & Immunology, Loyola University Chicago, Maywood, Illinois, United States
  • Charles S Bouchard
    Ophthalmology, Loyola University Chicago, Maywood, Illinois, United States
  • Ping Bu
    Ophthalmology, Loyola University Chicago, Maywood, Illinois, United States
  • Shaozhen Zhao
    Tianjin Medical University Eye Hospital, Tianjin, China
  • Footnotes
    Commercial Relationships   hui liu, None; Frank Gambino, None; Taylor Keller, None; Yougang Zhai, None; Liang Qiao, None; Charles Bouchard, None; Ping Bu, None; Shaozhen Zhao, None
  • Footnotes
    Support  This work was supported by a grant from the Illinois Society for the Prevention of Blindness (ISPB), and funding from The Richard A. Perritt Charitable Foundation.
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 441. doi:
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      hui liu, Frank Gambino, Taylor Keller, Yougang Zhai, Liang Qiao, Charles S Bouchard, Ping Bu, Shaozhen Zhao; Anti-inflammation effects of Zidovudine in human corneal epithelial cells. Invest. Ophthalmol. Vis. Sci. 2017;58(8):441.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Dry eye disease (DED) is a common ocular surface inflammatory disease, which can significantly impact the quality of life of patients. Activation of NLRP3 inflammasome and downstream casepase-1 and IL-1β activation and maturation play critical roles in the pathophysiology of DED. The purpose of this study is to determine the effect of Zidovudine (AZT), a nucleoside reverse transcriptase inhibitor, on inflammasome activation in corneal epithelial cells, a hyperosmolarity dry eye model.

Methods : Immortalized human corneal epithelial cells (HCECs) were pretreated in the presence or absence of 100mM and 200mM of AZT for 2 hours. HCECs were then exposed to media ranging from 312 mOsm (normal osmolarity) to 500 mOsm (hyperosmolarity). Cell culture supernatants were collected at 4, 12, and 24 hours. Epithelial cell viability and level of IL-1β were determined by a lactate dehydrogenase (LDH) assay and a specific capture ELISA (eBioscience).

Results : In the hyperosmolarity group, the LDH ratio and level of IL-1β were significantly increased as compared to the normal control osmolarity group. In the hyperosmolarity group pretreated with 100mM and 200mM AZT, the LDH ratio and IL-1β level were decreased as compared to non-AZT-treated hyperosmolarity group.

Conclusions : AZT can improve HCECs survival as evidenced by decreased LDH ratio and inhibit the production of the inflammatory cytokines IL-1β. Nucleoside reverse transcriptase inhibitors like AZT may be useful for the management of DED.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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