Purchase this article with an account.
Sang wook Choi, Bum Joo Cho, Kim Jeong Won, Tae-Young Chung, Joon-Young Hyon, Young Joo Shin; Rapamycin Prevents Endoplasmic Reticulum Stress-induced Dry Eye Syndrome in Mice. Invest. Ophthalmol. Vis. Sci. 2017;58(8):443.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To investigate whether rapamycin protect the tear and ocular surface against the endoplasmic reticulum (ER) stress-induced dry eye syndrome in mice.
Tunicamycin was injected intraperitoneally in balb/c mice without or with rapamycin (TM group or RM5 group). Phosphate buffered saline was injected intraperitoneally in control group. Blinking rate, fluorescein staining score (FSS) and phenol red-thread tear secretion test were measured at 4 days, 1 week, and 2 weeks after treatment. Levels of inflammatory and angiogenetic cytokines were measured by ELISA.
Blinking rate and FSS were elevated in TM group compared to control and tear secretion was decreased in TM group compared to control (p<0.05 for all), which was ameliorated by rapamycin at 1 week and at 2 weeks. Levels of inflammatory and angiogenetic cytokines in ocular surface and lacrimal glands were elevated in TM group compared to control and decreased in RM5 group compared to TM group at 1 week and at 2 weeks (p<0.05 for all).
Intraperitoneal injection of tunicamycin induced dry eye syndrome in mice. Rapamycin protected the tear and ocular surface against the ER stress-induced dry eye syndrome in mice through ameliorating ER stress-induced vascular damage and inflammation of lacrimal glands and ocular surface.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
This PDF is available to Subscribers Only