June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Rapamycin Prevents Endoplasmic Reticulum Stress-induced Dry Eye Syndrome in Mice
Author Affiliations & Notes
  • Sang wook Choi
    Ophthalmology, Hallym University Medical Center, Hallym University College of Medicine, Seoul, Korea (the Republic of)
  • Bum Joo Cho
    Ophthalmology, Hallym University Medical Center, Hallym University College of Medicine, Seoul, Korea (the Republic of)
  • Kim Jeong Won
    Pathology, Hallym University Medical Center, Hallym University College of Medicine, Seoul, Korea (the Republic of)
  • Tae-Young Chung
    Ophthalmology, Sungkyukwan University School of Medicine, Seoul, Korea (the Republic of)
  • Joon-Young Hyon
    Ophthalmology, Seoul National University Bundang Hospital , Seongnam, Gyeonggi, Korea (the Republic of)
    Ophthalmology, Seoul National University College of Medicine, Seoul, Korea (the Republic of)
  • Young Joo Shin
    Ophthalmology, Hallym University Medical Center, Hallym University College of Medicine, Seoul, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   Sang wook Choi, None; Bum Joo Cho, None; Kim Jeong Won, None; Tae-Young Chung, None; Joon-Young Hyon, None; Young Joo Shin, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 443. doi:
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      Sang wook Choi, Bum Joo Cho, Kim Jeong Won, Tae-Young Chung, Joon-Young Hyon, Young Joo Shin; Rapamycin Prevents Endoplasmic Reticulum Stress-induced Dry Eye Syndrome in Mice. Invest. Ophthalmol. Vis. Sci. 2017;58(8):443.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To investigate whether rapamycin protect the tear and ocular surface against the endoplasmic reticulum (ER) stress-induced dry eye syndrome in mice.

Methods : Tunicamycin was injected intraperitoneally in balb/c mice without or with rapamycin (TM group or RM5 group). Phosphate buffered saline was injected intraperitoneally in control group. Blinking rate, fluorescein staining score (FSS) and phenol red-thread tear secretion test were measured at 4 days, 1 week, and 2 weeks after treatment. Levels of inflammatory and angiogenetic cytokines were measured by ELISA.

Results : Blinking rate and FSS were elevated in TM group compared to control and tear secretion was decreased in TM group compared to control (p<0.05 for all), which was ameliorated by rapamycin at 1 week and at 2 weeks. Levels of inflammatory and angiogenetic cytokines in ocular surface and lacrimal glands were elevated in TM group compared to control and decreased in RM5 group compared to TM group at 1 week and at 2 weeks (p<0.05 for all).

Conclusions : Intraperitoneal injection of tunicamycin induced dry eye syndrome in mice. Rapamycin protected the tear and ocular surface against the ER stress-induced dry eye syndrome in mice through ameliorating ER stress-induced vascular damage and inflammation of lacrimal glands and ocular surface.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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