June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
The protective effect of a topical mucin secretagogue on the ocular surface damage induced by airborne black carbon exposure
Author Affiliations & Notes
  • Jong Suk Song
    Ophthalmology, Korea University College of Medicine, Seoul, Korea (the Republic of)
  • Boram Kang
    Ophthalmology, Korea University College of Medicine, Seoul, Korea (the Republic of)
  • Xiangzhe Lee
    Ophthalmology, Korea University College of Medicine, Seoul, Korea (the Republic of)
  • Youngsub Eom
    Ophthalmology, Korea University College of Medicine, Seoul, Korea (the Republic of)
  • Hyo Myung Kim
    Ophthalmology, Korea University College of Medicine, Seoul, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   Jong Suk Song, None; Boram Kang, None; Xiangzhe Lee, None; Youngsub Eom, None; Hyo Myung Kim, None
  • Footnotes
    Support  A grant from the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (Grant No: HI13C0055)
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 451. doi:
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    • Get Citation

      Jong Suk Song, Boram Kang, Xiangzhe Lee, Youngsub Eom, Hyo Myung Kim; The protective effect of a topical mucin secretagogue on the ocular surface damage induced by airborne black carbon exposure. Invest. Ophthalmol. Vis. Sci. 2017;58(8):451.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Exposure to airborne particulate matter can induce ocular surface damage and inflammation. We evaluated the effects of a topical mucin secretagogue on the protection of ocular surface damage induced by exposure to airborne black carbon, which is one of major airborne particulate matter components.

Methods : Lewis Rats were exposed to ambient black carbon for two hours twice daily for five days. Corneal staining score and tear lactic dehydrogenase (LDH) activity were measured to evaluate ocular surface damage and compared with normal controls. Serum immunoglobulin (Ig) G and E were assayed using enzyme-linked immunosorbent assay, and the size of cervical lymph nodes was measured. The expression of interleukin (IL)-4, 17, and interferon (IFN)-γ in the anterior segment of the eyeball and cervical lymph nodes was measured by western blot analysis. Diquafosol tetrasodium was instilled six times a day from one day before exposure to five-day exposure duration and the ocular surface damages were evaluated and compared with saline instillation.

Results : After exposure to airborne black carbon exposure, the median corneal staining scores (0.75) and LDH activity (0.63 optical density [OD]) were significantly increased compared with those of normal controls (0, 0.44, respectively) (n=6). Serum IgG and IgE levels and the size of cervical lymph nodes were significantly increased. The expression of IL-4, IL-17, IFN-γ were elevated in the anterior segment of the eyeball and cervical lymph nodes. When exposed to airborne black carbon, topical diquafosol tetrasodium significantly increased tear MUC5AC concentration (median value, 5.83 ng/ml) and decreased tear LDH activity (median value, 0.48 OD) compared with saline instillation (2.29 ng/ml, 0.59 OD) (n=4).

Conclusions : Exposure to airborne black carbon induced ocular surface and increased pro-inflammatory cytokines in the eyes and cervical lymph nodes. Topical mucin secretagogues seem to have a protective effect on the ocular surface from the exposure to airborne particulate matters.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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