June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Caspase-8 promotes the activation of NLRP3 inflammasome and inhibits the production of NLRP6 in dry eye disease
Author Affiliations & Notes
  • Yonghao Li
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Wei Chi
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China
  • Footnotes
    Commercial Relationships   Yonghao Li, None; Wei Chi, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 459. doi:
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      Yonghao Li, Wei Chi; Caspase-8 promotes the activation of NLRP3 inflammasome and inhibits the production of NLRP6 in dry eye disease. Invest. Ophthalmol. Vis. Sci. 2017;58(8):459.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Dry eye disease (DED) is the most common ocular surface disease, average one out of every five persons suffered by this disease in the worldwide. Accumulating evidence demonstrates that immunoinflammatory mechanisms play pivotal roles in the pathogenesis of DED. However, the potential roles and mechanism of innate immune are largely unknown.

Methods : In the present study, we used an experimental murine dry eye model and in vitro human limbal epithelial cell (HLEC) dry eye model induced by hyperosmotic stress to investigate the underlying mechanisms of innate immune in DED.

Results : We found that caspase-8 activated by ROS play a key role in the pathogenesis of DED by forming a caspase-8-ASC inflammasome, promoting the activation of NLRP3 inflammasome and downregulating NLRP6 inflammasome via NF-κB pathway. The activation of NLRP3 inflammasome can also suppress the production of NLRP6 and promote the processing of IL-1β and IL-18, actively involving in the innate immune responses of DED.

Conclusions : These findings demonstrate collectively a critical role of caspase-8-inducing NLRP3 inflammasome activation in processing IL-1β and IL-18 maturation and decreasing the expression of NLRP6 in DED. These results provide new insight into the pathogenesis of DED and point to a treatment strategy.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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