June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Ultraviolet irradiation induces the secretion of IL-1β via activation of NLRP3 inflammasomes in human retinal pigment epithelial cells
Author Affiliations & Notes
  • Eveliina Korhonen
    University of Eastern Finland, Kuopio, Finland
  • Niina Piippo
    University of Eastern Finland, Kuopio, Finland
  • Maria Hytti
    University of Eastern Finland, Kuopio, Finland
  • Kai Kaarniranta
    University of Eastern Finland, Kuopio, Finland
    Kuopio University Hospital, Kuopio, Finland
  • Anu Kauppinen
    University of Eastern Finland, Kuopio, Finland
  • Footnotes
    Commercial Relationships   Eveliina Korhonen, None; Niina Piippo, None; Maria Hytti, None; Kai Kaarniranta, None; Anu Kauppinen, None
  • Footnotes
    Support  Alfred Kordelin Foundation, Finnish Cultural Foundation, Academy of Finland (grants 297267 and 307341), Kuopio University Hospital, Silmäsäätiö, Emil Aaltonen Foundation, Silmä- ja Kudospankkisäätiö, Sokeain Ystävät ry
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 605. doi:
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    • Get Citation

      Eveliina Korhonen, Niina Piippo, Maria Hytti, Kai Kaarniranta, Anu Kauppinen; Ultraviolet irradiation induces the secretion of IL-1β via activation of NLRP3 inflammasomes in human retinal pigment epithelial cells. Invest. Ophthalmol. Vis. Sci. 2017;58(8):605.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Formation of inflammasome complexes are required for maturation and release of early initiator of immune responses, interleukin (IL)-1β. The aim of the present study was to investigate whether ultraviolet (UV) irradiation can activate nod-like receptor protein 3 (NLRP3) inflammasomes and further induce secretion of IL-1β in human retinal pigment epithelial (ARPE-19) cells in vitro. In addition, the role of potassium efflux as an upstream mechanism of inflammasome activation was explored in the present study.

Methods : ARPE-19 cells were primed with IL-1α and treated with potassium chloride prior to irradiation of UV light (wavelength of 290-320 nm). For siRNA experiments, subconfluent cell layers were transfected with NLRP3 siRNA prior to priming signal. Secretion of IL-1β and cleaved caspase-1 as well as levels of NLRP3 were detected by ELISA assay. All data was analyzed by using Mann-Whitney U-test.

Results : IL-1α primed ARPE-19 cells upon UV irradiation induced secretion of IL-1β (p<0.001), which was further declined by preventing potassium efflux during the UV exposure (p<0.001). Furthermore, significantly increased levels of NLRP3 and cleaved caspase-1 were found from cell culture medium after UV exposure (both p<0.001), indicating that inflammasome components were released out from the cells after inflammasome activation. In addition, NLRP3 siRNA revealed decreased release of IL-1β compared to negative siRNA control (p<0.01).

Conclusions : Our data shows that UV activates NLRP3 inflammasomes, which further triggers the secretion of IL-1β in ARPE-19 cells. In addition, potassium efflux seems to be involved in UV induced inflammasome activation.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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