June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Retina-specific molecular species (PC and PE) alterations by ablation of AdipoR1 or MFRP
Author Affiliations & Notes
  • Bokkyoo Jun
    Neuroscience Center, LSU Health Sciences Center, New Orleans, Louisiana, United States
  • Marie-Audrey Ines Kautzmann
    Neuroscience Center, LSU Health Sciences Center, New Orleans, Louisiana, United States
  • Helen Elizabeth Hill
    Neuroscience Center, LSU Health Sciences Center, New Orleans, Louisiana, United States
  • Uday B. Patel
    Neuroscience Center, LSU Health Sciences Center, New Orleans, Louisiana, United States
  • William C Gordon
    Neuroscience Center, LSU Health Sciences Center, New Orleans, Louisiana, United States
  • Nicolas Guillermo Bazan
    Neuroscience Center, LSU Health Sciences Center, New Orleans, Louisiana, United States
  • Footnotes
    Commercial Relationships   Bokkyoo Jun, None; Marie-Audrey Kautzmann, None; Helen Hill, None; Uday Patel, None; William Gordon, None; Nicolas Bazan, None
  • Footnotes
    Support  Supported by NEI grant EY005121, NIGMS grant GM103340, the Eye, Ear, Nose and Throat Foundation (NGB), and Research to Prevent Blindness
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 632. doi:
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    • Get Citation

      Bokkyoo Jun, Marie-Audrey Ines Kautzmann, Helen Elizabeth Hill, Uday B. Patel, William C Gordon, Nicolas Guillermo Bazan; Retina-specific molecular species (PC and PE) alterations by ablation of AdipoR1 or MFRP. Invest. Ophthalmol. Vis. Sci. 2017;58(8):632.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We have shown that retinas of adiponectin receptor 1 (AdipoR1) knockout (KO) mice and membrane frizzled-related protein (MFRP) KO mice have reduced docosahexaenoic acid (DHA) and very-long-chain polyunsaturated fatty acids (VLC-PUFAs) contained in phoshatidylcholine (PC) molecular species. This led to retina degeneration. DHA is enriched in retina, and AdipoR1 and MFRP are involved in DHA uptake/retention in the retina, so we asked if molecular distribution of fatty acids in phosphatidylethanolamine (PE) follows a similar behavior. We also asked if PC and PE molecular species are affected by AdipoR1 KO and MFRP KO in a retina-specific fashion.

Methods : Lipids were extracted from retinas, brains, kidneys, livers and hearts from AdipoR1 KO, MFRP KO, and control mice. Liquid chromatography tandem mass spectrometry (LC-MS/MS) was used for the measurement of PC and PE molecular species. Naturally-occurring isotope correction was done using self-made programs before distribution of PC and PE was calculated.

Results : In retina, both AdipoR1 KO and MFRP KO showed reduction of DHA-containing PC and PE, especially PC(44:12) and PE(44:12), which contain DHA for both sn1 and sn2. VLC-PUFAs (ranging from 32 to 38 carbons) occurred only in PCs. Arachidonic acid(AA)-containing PC and PE (PC(36:4),PE(38:4)) increased in the KO retinas, indicating AA replaced DHA in PC and PE. However, other organs did not show any differences among the groups.

Conclusions : The reduction of DHA and VLC-PUFAs in AdipoR1 KO or MFRP KO in PC and PE molecular species is retina-specific. Even though AdipoR1 is expressed in other organs, including kidney, liver and brain, DHA uptake/retention in those organs was not affected by AdipoR1/MFRP ablation.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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