June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Inhibition of Corneal Neovascularization by Dexamethasone-Eluting Contact Lenses in a Rabbit Model
Author Affiliations & Notes
  • Hidenaga KOBASHI
    Ophthalmology, Schepens Eye Research Institute, Brookline, Massachusetts, United States
  • Lokendra Bengani
    Ophthalmology, Schepens Eye Research Institute, Brookline, Massachusetts, United States
  • Amy E. Ross
    Ophthalmology, Schepens Eye Research Institute, Brookline, Massachusetts, United States
  • Hualei Zhai
    Ophthalmology, Schepens Eye Research Institute, Brookline, Massachusetts, United States
  • Sharad Mittal
    Ophthalmology, Schepens Eye Research Institute, Brookline, Massachusetts, United States
  • Sunil Chauhan
    Ophthalmology, Schepens Eye Research Institute, Brookline, Massachusetts, United States
  • Daniel S. Kohane
    Department of Anesthesiology, Boston Children’s Hospital, Boston, Massachusetts, United States
  • Joseph B Ciolino
    Ophthalmology, Schepens Eye Research Institute, Brookline, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Hidenaga KOBASHI, None; Lokendra Bengani, None; Amy E. Ross, None; Hualei Zhai, None; Sharad Mittal, None; Sunil Chauhan, None; Daniel S. Kohane, Boston Children’s Hospital (P); Joseph Ciolino, Mass Eye and Ear (P)
  • Footnotes
    Support  1) Department of Defense 2) Research to prevent blindness 2) MEEI/BLJ Ocular Surface Research Fellowship
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 1000. doi:
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    • Get Citation

      Hidenaga KOBASHI, Lokendra Bengani, Amy E. Ross, Hualei Zhai, Sharad Mittal, Sunil Chauhan, Daniel S. Kohane, Joseph B Ciolino; Inhibition of Corneal Neovascularization by Dexamethasone-Eluting Contact Lenses in a Rabbit Model. Invest. Ophthalmol. Vis. Sci. 2017;58(8):1000.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To evaluate the effects of dexamethasone-eluting contact lenses (D-lenses) on experimentally induced corneal neovascularization (CNV) and to compare the efficacy to that of the current mainstay of treatment, which is the frequent use of steroid eye drops.

Methods : CNV was induced by placing 7-0 silk sutures along the superior and inferior corneas of the right eye of New Zealand white rabbits, which were then exposed to one of the following regimens for one week: 1) no Treatment, 2) topical dexamethasone sodium phosphate drops (0.1 %, 8 drops a day), 3) D-lenses, 4) Contact lenses without dexamethasone (n = 6 per group). Two masked observers measured the area of CNV (mm2) from slit lamp photographs taken on day 7. Corneal inflammation was assessed on day 7 by analyzing CD45+ cell (leukocyte) frequencies in corneal tissue using flow cytometry.

Results : CNV area (mm2) was less for D-Lens (4.0 ± 2.2) compared to no treatment (15.0 ± 3.2, p < 0.001) or contact lenses without dexamethasone (17.6 ± 2.6, p < 0.001), but not significantly different from hourly dexamethasone drops (3.4 ± 2.8, p=0.966). The frequencies of CD45+ cells in corneal tissues were significantly lower in D-Lens (5.0 ± 4.5) group compared to no treatment (14.8 ± 4.1, p < 0.001) or contact lens without dexamethasone (10.5 ± 4.0, p = 0.023) group, but were not different from dexamethasone hourly drop group (4.8 ± 2.7, p=0.942).

Conclusions : Dexamethasone-eluting contact lens effectively inhibited CNV and corneal inflammation, similar to the efficacy of hourly-administred dexamethasone eye drops. Contact lens drug delivery may be an option for the prevention of post-keratoplasty rejection and a platform for ocular drug delivery.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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