June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Pharmacogenetic manipulation of neuronal activity reveals a role of brain spinal trigeminal nucleus in reflex tearing.
Author Affiliations & Notes
  • Yusuke Izuta
    Ophthalmology, School of Medicine, Keio Univetsity, Shinjuku, Japan
  • Michiko Shibuya
    Ophthalmology, School of Medicine, Keio Univetsity, Shinjuku, Japan
  • Erina Onishi
    Ophthalmology, School of Medicine, Keio Univetsity, Shinjuku, Japan
  • Imada Toshihiro
    Ophthalmology, School of Medicine, Keio Univetsity, Shinjuku, Japan
  • Shigeru Nakamura
    Ophthalmology, School of Medicine, Keio Univetsity, Shinjuku, Japan
  • Ayano Katagiri
    Department of Physiology, Nihon University School of Dentistry, Tokyo, Japan
  • Akihiro Yamanaka
    Department of Neuroscience II, Nagoya University Research Institute of Environmental Medicine, Nagoya, Japan
  • Kazuo Tsubota
    Ophthalmology, School of Medicine, Keio Univetsity, Shinjuku, Japan
  • Footnotes
    Commercial Relationships   Yusuke Izuta, None; Michiko Shibuya, None; Erina Onishi, None; Imada Toshihiro, None; Shigeru Nakamura, None; Ayano Katagiri, None; Akihiro Yamanaka, None; Kazuo Tsubota, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 1012. doi:
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      Yusuke Izuta, Michiko Shibuya, Erina Onishi, Imada Toshihiro, Shigeru Nakamura, Ayano Katagiri, Akihiro Yamanaka, Kazuo Tsubota; Pharmacogenetic manipulation of neuronal activity reveals a role of brain spinal trigeminal nucleus in reflex tearing.. Invest. Ophthalmol. Vis. Sci. 2017;58(8):1012.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Reflex tearing, marked transient secretion, is physiological reaction against sensory stimuli to cornea from external. Vc/C1 region of spinal trigeminal nucleus of brain (Vc/C1) is known to relay region of corneal sensation. Designer Receptors Excusively Activated by Designer Drugs (DREADD) technology enable to manipulate specific neuronal activity in a freely moving animal. In this study, using DREADD system, we investigate the role of the Vc/C1 in reflex tearing.

Methods : Eight weeks, female C57Bl/6J mice were used. Surgeries for Adeno associated virus (AAV) injections were conducted under pentobarbital anesthesia (50 mg/kg, i.p.) using stereotaxic instrument with a glass micropipette and an air pressure injector system. AAV-CMV-hM3Dq-mCherry (600nl/injection) were unilaterally injected into Vc/C1 (according to mouse brain atlas). Two weeks over after the surgery, the changes in tear secretion and eye scratching behavior were analyzed after the fiting of hM3Dq expressed neurons by administration of hM3Dq agonist clozapine-N-oxide (CNO) (1mg/kg, i.p.). Saline was used as control.

Results : Increase in tear secretion along with eye scratching behavior were specifically observed in ipsilateral to the AAV injected side Increasing ratio were peaked at 30min after the CNO injection, approximately 200% (tear secretion) and 3000% (eye scratching) to before CNO injection (n=5). These increase were sustained up to 240minutes. Administration of saline did not altered these responses. In all mice, the expresiion of hM3Dq-mCherry were confirmed in Vc/C1 region.

Conclusions : Our results indicate that activation of Vc/C1 region involves in reflex tearing.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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