June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Potential Role of IL-10-producing Th17 Cells in Pathogenesis of Dry Eye Disease
Author Affiliations & Notes
  • Qi Hong
    Peking University Eye Center, Peking University Third Hospital, Beijing, China
    Department of Ophthalmology, Schepens EyeResearch Institute, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States
  • Yihe Chen
    Department of Ophthalmology, Schepens EyeResearch Institute, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States
  • Takenori Inomata
    Department of Ophthalmology, Schepens EyeResearch Institute, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States
  • Rongjun Liu
    Peking University Eye Center, Peking University Third Hospital, Beijing, China
  • Reza Dana
    Department of Ophthalmology, Schepens EyeResearch Institute, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Qi Hong, None; Yihe Chen, None; Takenori Inomata, None; Rongjun Liu, None; Reza Dana, None
  • Footnotes
    Support  National Natural Science Foundation of China (No. 30872813); the grant of China Scholarship Council; the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry; EY 20889; NIH R01 EY 20889 grant
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 1022. doi:
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    • Get Citation

      Qi Hong, Yihe Chen, Takenori Inomata, Rongjun Liu, Reza Dana; Potential Role of IL-10-producing Th17 Cells in Pathogenesis of Dry Eye Disease. Invest. Ophthalmol. Vis. Sci. 2017;58(8):1022.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To analyze the expression of Interleukin (IL)-17 in the induction and abatement phases of dry eye disease (DED), and to explore the potential role of IL-10-producing Th17 cells in disease pathogenesis using a mouse model of DED.

Methods : DED was induced in female C57BL/6 mice. Mice exposed to desiccating stress in a controlled environment chamber for 14 consecutive days were used as the acute DED group, and mice that were transferred to a standard environment and maintained for an additional 14 days after DED induction were used as the chronic DED group. Untreated mice were used as normal controls. Corneal fluorescein staining (CFS) was performed to assess severity of DED.The expression levels of IL-17 in conjunctiva and cervical draining lymph nodes (DLNs) were measured using real-time PCR and ELISA. The frequencies of CD4+ IL-17+ (Th17) cells and IL-10-producingTh17 cells in conjunctiva and DLNs were evaluated with flow cytometry.

Results : The CFS scores, mRNA and protein expression levels of IL-17, and the frequencies of Th17 cells in both conjunctiva and DLNs were significantly higher in acute DED group compared to relived DED group and non-DED controls (p<0.05). Interestingly, the frequencies of IL-10-producing Th17 cells (CD4+IL-17+IL-10+) were significantly lower in the conjunctivae and DLNs of acute DED group compared to non-DED controls (p<0.05). Furthermore, mice with chronic DEDshowed significantly higher frequencies of IL-10-producing Th17 cells in the conjunctivae compared to mice with acute DED (p<0.01).

Conclusions : Our findings demonstrate that IL-10-producing Th17 cells may play a regulatory role in DED.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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