June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
The Effects of Electromagnetic Fields on Cultured Human Retinal Pigment Epithelial Cells
Author Affiliations & Notes
  • Mozhgan Rezaeikanavi
    Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran (the Islamic Republic of)
  • Niyousha Nasrabadi
    Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran (the Islamic Republic of)
  • Zahra-Soheila Soheili
    National Institute of Genetic Engineering and Biotechnology, Tehran, Iran (the Islamic Republic of)
  • Abouzar Bagheri
    University of Social Welfare and Rehabilitation Sciences , Tehran, Iran (the Islamic Republic of)
    Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran (the Islamic Republic of)
  • Footnotes
    Commercial Relationships   Mozhgan Rezaeikanavi, None; Niyousha Nasrabadi, None; Zahra-Soheila Soheili, None; Abouzar Bagheri, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 1057. doi:
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      Mozhgan Rezaeikanavi, Niyousha Nasrabadi, Zahra-Soheila Soheili, Abouzar Bagheri; The Effects of Electromagnetic Fields on Cultured Human Retinal Pigment Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2017;58(8):1057.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : A great deal of evidence has confirmed that electromagnetic fields can affect the central nervous system and are effective in gene expression as well as development, differentiation, cell proliferation, and apoptosis of embryonic neural cells. This study was conducted to investigate the cellular and molecular changes of cultured human retinal pigment epithelial (hRPE) cells when treated with pulsed electromagnetic fields.

Methods : Cultured neonatal hRPE cells with confluency of 60% were exposed to pulsed electromagnetic field of 1 mT intensity and 50 Hz frequency 8 hours daily for 3 days. In addition to cell proliferation and cell death assays, immunocytochemistry for RPE65, Pax6, Nestin and cytokeratin 8/18 proteins were performed. Extracted RNAs were subjected to real-time PCR for Nestin, Pax6, RPE65, and α-SMA.

Results : Treated hRPE cells did not demonstrate significant change in terms of cell proliferation and cell death. Protein expressions of Pax6, Nestin, and cytokeratin 8/18 were decreased in treated cells compared to controls and remained unchanged for RPE65. Gene expressions of Nestin, RPE65 and Pax6 were decreased in treated cells as compared to controls (P value < 0.05). Gene expression of α-SMA did not reveal a significant change.

Conclusions : Our study demonstrated that both gene and protein expressions of retinal progenitor cells markers were decreased in cultivated hRPE cells after exposure to safe dose of pulsed electromagnetic field. Decreased protein expression of cytokeratin 8/18 and decreased gene expression of RPE65 may be indicative of either transdifferentiation or senescence of RPE cells.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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