June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
The Effect of Human Corneal-Derived Mesenchymal Stromal Cells Secretome on the Human Macrophages Viability
Author Affiliations & Notes
  • Ilham Putra
    Ophthalmology and Vision Science, University of Illinois- Chicago, Chicago, Illinois, United States
  • Medi Eslani
    Ophthalmology and Vision Science, University of Illinois- Chicago, Chicago, Illinois, United States
  • Judy Hamouie
    Ophthalmology and Vision Science, University of Illinois- Chicago, Chicago, Illinois, United States
  • Asha Tadepalli
    Ophthalmology and Vision Science, University of Illinois- Chicago, Chicago, Illinois, United States
  • Xiang Shen
    Ophthalmology and Vision Science, University of Illinois- Chicago, Chicago, Illinois, United States
  • Vivek Desai
    Ophthalmology and Vision Science, University of Illinois- Chicago, Chicago, Illinois, United States
  • Peiman Hematti
    Medicine, Hematology/Oncology division, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, United States
  • Ali R Djalilian
    Ophthalmology and Vision Science, University of Illinois- Chicago, Chicago, Illinois, United States
  • Footnotes
    Commercial Relationships   Ilham Putra, None; Medi Eslani, None; Judy Hamouie, None; Asha Tadepalli, None; Xiang Shen, None; Vivek Desai, None; Peiman Hematti, None; Ali Djalilian, None
  • Footnotes
    Support  Clinical Scientist Development Program Award K12EY021475 (ME), R01 EY024349-01A1 (ARD) and Core grant EY01792 from NEI/NIH; MR130543 (ARD) from DoD, Vision for Tomorrow (ARD), unrestricted grant to the department from RPB; and Eversight (providing both seed funding and human corneal research tissue).
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 981. doi:
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    • Get Citation

      Ilham Putra, Medi Eslani, Judy Hamouie, Asha Tadepalli, Xiang Shen, Vivek Desai, Peiman Hematti, Ali R Djalilian; The Effect of Human Corneal-Derived Mesenchymal Stromal Cells Secretome on the Human Macrophages Viability. Invest. Ophthalmol. Vis. Sci. 2017;58(8):981.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The anti-inflammatory properties of mesenchymal stromal/stem cells (MSCs) have been recognized as one of their major modes of action. Macrophages (Mq) are the key player of the innate immune system in the most stages of inflammation. We evaluated the effect of the human corneal limbal derived- MSCs (CL-MSCs) secretome on the viability of human Mqs.

Methods : CL- MSCs were isolated from healthy cadaver donors and cultured in MEM-alpha media with 10% fetal bovine serum. Their secretome were collected in serum-free media after 48 hours. CD14+ cells were isolated from peripheral blood mononuclear cells and were differentiated into Mqs in IMDM + 10% serum. ApoTox-Glo™ Triplex Assay (Promega) was used to determine viability, cytotoxicity and apoptosis of Mqs after exposure to the CL-MSCs secretome.

Results : There was not a statistical difference in viability and cytotoxicity of Mqs 8 hours after exposure to the CL-MSCs secretome compared to the unconditioned media (P > 0.05 for both). However, there was a 2.4 ± 5.35 folds increase in apoptosis rate of Mqs 8 hours after incubation with CL-MSCs secretome compared to the control (P <0.001).

Conclusions : Inducing apoptosis in the Mqs may be one of the mechanisms that CL-MSCs confer their anti-inflammatory property.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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