June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
The Effect of Lipopolysaccharide on TSG6 Expression in Mesenchymal Stromal Cells
Author Affiliations & Notes
  • Gaurav Agnihotri
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Samaneh Ghassemi
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Ilham Putra
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Xiang Shen
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Jigna Sheth
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Medi Eslani
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Peiman Hematti
    Hematology/oncology, Medicine, University of Wisconsin at Madison, Madison, Wisconsin, United States
  • Ali R Djalilian
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Footnotes
    Commercial Relationships   Gaurav Agnihotri, None; Samaneh Ghassemi, None; Ilham Putra, None; Xiang Shen, None; Jigna Sheth, None; Medi Eslani, None; Peiman Hematti, None; Ali Djalilian, None
  • Footnotes
    Support  Clinical Scientist Development Program Award K12EY021475 (ME), R01 EY024349-01A1 (ARD) and Core grant EY01792 from NEI/NIH; MR130543 (ARD) from DoD, Vision for Tomorrow (ARD), unrestricted grant to the department from RPB; and Eversight (providing both seed funding and human corneal research tissue).
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 991. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Gaurav Agnihotri, Samaneh Ghassemi, Ilham Putra, Xiang Shen, Jigna Sheth, Medi Eslani, Peiman Hematti, Ali R Djalilian; The Effect of Lipopolysaccharide on TSG6 Expression in Mesenchymal Stromal Cells. Invest. Ophthalmol. Vis. Sci. 2017;58(8):991.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Mesenchymal stem cells (MSCs) play an important role during inflammation and its resolution. We evaluated the effect of lipopolysaccharides (LPS) found in gram-negative bacteria on TSG6 expression (a well-known anti-inflammatory agent) in the corneal-limbal and bone-marrow derived MSCs (CL-MSC and BM-MSC).

Methods : BM-MSC were isolated from human bone marrow donors in a GMP facility. CL-MSCs were extracted from healthy cadaver corneas. MSCs were grown to confluency using MEM-alpha + 10% fetal bovine serum. Telomerase-immortalized human corneal epithelial cells (HCEC) were used as an external control to MSCs. They were stimulated with 100 ng/ml LPS for 24 hours, followed by mRNA extraction. After reverse transcription, the mRNA expression of TSG6 was analyzed with delta-deltaCt method by real-time qPCR using GAPDH as control.

Results : LPS treatment resulted in a 4.7 and 5.78 folds increase in TSG6 mRNA expression in CL-MSCs and BM-MSCs compared to the unstimulated cells, respectively (P < 0.01). There were no significant changes in TSG6 mRNA expression in HCECs after incubation with LPS.

Conclusions : LPS exposure leads to upregulation of TSG6 in MSCs. This response is observed in MSCs and not HCECs. Protein expression is needed to confirm these results.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×