June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Axotomy-induced retinal ganglion cell death is attenuated by pluripotent cells isolated from the retinal ciliary body.
Author Affiliations & Notes
  • Fernando Lucas-Ruiz
    Experimental Ophthalmology, IMIB-Arrixaca, El Palmar, Murcia, Spain
  • Marta Fernández-Nogales
    Instituto de Neurociencias (CSIC-UMH), Alicante, Spain
  • F Javier Valiente-Soriano
    Experimental Ophthalmology, IMIB-Arrixaca, El Palmar, Murcia, Spain
  • Francisco M Nadal-Nicolas
    Experimental Ophthalmology, IMIB-Arrixaca, El Palmar, Murcia, Spain
  • Diana Baeza
    Instituto de Neurociencias (CSIC-UMH), Alicante, Spain
  • Eloisa Herrera González de Molina
    Instituto de Neurociencias (CSIC-UMH), Alicante, Spain
  • Marta Agudo-Barriuso
    Experimental Ophthalmology, IMIB-Arrixaca, El Palmar, Murcia, Spain
  • Footnotes
    Commercial Relationships   Fernando Lucas-Ruiz, None; Marta Fernández-Nogales, None; F Javier Valiente-Soriano, None; Francisco Nadal-Nicolas, None; Diana Baeza, None; Eloisa Herrera González de Molina, None; Marta Agudo-Barriuso, None
  • Footnotes
    Support  Fundació La Marató de TV3 (MALALTIES NEURODEGENERATIVES 2013, PROJECTE 20142130/20142131)
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 1366. doi:
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      Fernando Lucas-Ruiz, Marta Fernández-Nogales, F Javier Valiente-Soriano, Francisco M Nadal-Nicolas, Diana Baeza, Eloisa Herrera González de Molina, Marta Agudo-Barriuso; Axotomy-induced retinal ganglion cell death is attenuated by pluripotent cells isolated from the retinal ciliary body.. Invest. Ophthalmol. Vis. Sci. 2017;58(8):1366.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To investigate the therapeutic potential of pluripotent cells isolated from the ciliary body (CBCs) of adult retinas on the survival of retinal ganglion cells (RGCs) after axonal injury.

Methods : CBCs isolated from C57/Bl6 adult mice were administered into the vitreous body of mice right after performing optic nerve crush. Two additional groups were used as controls: a group injected only with the vehicle (HBSS) and another group injected with CBCs into intact retinas (n=6-10/group). Axotomized and control retinas were analyzed at different time points after the lesion and the number of surviving RGCs was quantified.

Results : In control retinas we observed that vehicle or CBCs are not toxic for RGCs, nor they divide or form tumors. Compared to vehicle, treatment with CBCs increased the survival of axotomized RGCs at all time points, being the beneficial effect more apparent at longer time periods, reaching up to an 83% at 14 days and 70% at 30 days more survival than in vehicle-treated retinas.

Conclusions : The injection of CBCs in optic nerve axotomized mice delays RGCs death suggesting that these cells may have a neuroprotective effect after axonal injury.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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