June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Effect of Advanced Glycation End Products on the generation of ROS in Corneal Epithelial Cells
Author Affiliations & Notes
  • Long Shi
    Ophthalmology, Qilu Hospital of Shandong University, JINAN, SHANDONG, China
  • xy wu
    Ophthalmology, Qilu Hospital of Shandong University, JINAN, SHANDONG, China
  • Footnotes
    Commercial Relationships   Long Shi, None; xy wu, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 1374. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Long Shi, xy wu; Effect of Advanced Glycation End Products on the generation of ROS in Corneal Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2017;58(8):1374.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To investigate the effect of Advanced Glycation End Products (AGE) on the generation of reactive oxygen species (ROS) in corneal epithelial cells and the underlying mechanisms in vitro.

Methods : Telomerase-immortalized human corneal epithelial cells (THCEs) were treated with AGE-modified bovine serum albumin (BSA) for various times. The production of ROS was measured with 2', 7'- dichlorofluorescein diacetate (DCFH-DA) dye assay and imaged on laser scanning confocal fluorescence microscope. NADPH oxidase activity was measured by luminescence assay. The protein levels of p22phox and Nox4 were determined by Western blot.

Results : AGE-BSA significantly increased the generation of intracellular ROS in THCEs cells. However, the generation of intracellular ROS was completely inhibited by antioxidant N-acetylcysteine (NAC), anti-receptor of AGEs (RAGE) antibodies, or the inhibitor of NADPH oxidase. Moreover, AGE-BSAincreased NADPH oxidase activity and protein expression of NADPH oxidase subunits, p22phox and Nox4, but anti-RAGE antibodies eliminated these effects.

Conclusions : In conclusion, our results demonstrated that AGE-BSA increased intracellular ROS generation through NADPH oxidase activation, which may account for the delayed corneal epithelial wound healing. These results may provide better insights for understanding the mechanism of delayed healing of corneal epithelial wounds in diabetes.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×