June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Corneal expression pattern of Semaphorin 3F
Author Affiliations & Notes
  • Tristan Reuer
    Eye Center, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany
  • Bertan Cakir
    Eye Center, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany
  • Felix Bock
    Department of Ophthalmology, University of Cologne, Cologne, Germany
  • Anima D Buehler
    Eye Center, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany
  • Johanna Madeleine Walz
    Eye Center, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany
  • Clemens Lange
    Eye Center, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany
  • Hansjuergen Agostini
    Eye Center, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany
  • Gunther R Schlunck
    Eye Center, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany
  • Claus Cursiefen
    Department of Ophthalmology, University of Cologne, Cologne, Germany
  • Thomas Reinhard
    Eye Center, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany
  • Andreas Stahl
    Eye Center, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany
  • Footnotes
    Commercial Relationships   Tristan Reuer, None; Bertan Cakir, None; Felix Bock, None; Anima Buehler, None; Johanna Madeleine Walz, None; Clemens Lange, None; Hansjuergen Agostini, None; Gunther Schlunck, None; Claus Cursiefen, None; Thomas Reinhard, None; Andreas Stahl, None
  • Footnotes
    Support  DFG FOR2240 , DFG STA 1102/5-1
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 1417. doi:
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    • Get Citation

      Tristan Reuer, Bertan Cakir, Felix Bock, Anima D Buehler, Johanna Madeleine Walz, Clemens Lange, Hansjuergen Agostini, Gunther R Schlunck, Claus Cursiefen, Thomas Reinhard, Andreas Stahl; Corneal expression pattern of Semaphorin 3F. Invest. Ophthalmol. Vis. Sci. 2017;58(8):1417.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Semaphorins are known modulators of angiogenesis. In the retina, semaphorin 3F (Sema3F) is selectively expressed in the outer nuclear layer and retinal pigment epithelium. Retinal Sema3F expression thus co-localizes with the physiologically avascular zone in the outer retina. Previous work from our group and others have demonstrated an anti-angiogenic effect of Sema3F. We have thus investigated whether Sema3F expression might co-localize with other physiologically avascular ocular tissues such as the cornea.

Methods : Sema3F expression in murine and human corneas was analyzed using qPCR and immunohistochemistry. In addition, Sema3F expression was quantified in primary human corneal epithelial cells in vitro using qPCR and Western Blot.

Results : Quantitative PCR revealed Sema3F expression in both murine and human corneas. Corneal Sema3F expression was significantly higher compared to expression in the adjacent conjunctiva. Within the cornea, there was no difference in Sema3F expression between central and peripheral regions. Immunohistochemistry localized Sema3F protein in the cornea mainly to the epithelium. In concordance, isolated primary human corneal epithelial cells expressed Sema3F both on mRNA as well as protein levels.

Conclusions : Similar to the physiologically avascular outer retinal layers, Sema3F is strongly expressed in corneal epithelium. Importantly, Sema3F expression is high in the cornea and almost undetectable in adjacent conjunctiva, indicating a selective expression in physiologically avascular tissues. This was confirmed both on mRNA as well as protein levels. These data suggest a potential role for Sema3F in maintaining corneal tissue avascularity.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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