June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Shp2 protein tyrosine phosphatase affects corneal epithelial stratification and innervation
Author Affiliations & Notes
  • Yuka Okada
    School of Optometry, Indian University, Bloomington, Indiana, United States
    Ophthalmology, Wakayama Medical University, Wakayama, Japan
  • Yujin Zhang
    School of Optometry, Indian University, Bloomington, Indiana, United States
  • Lingling Zhang
    School of Optometry, Indian University, Bloomington, Indiana, United States
  • Yen-Chiao Wang
    School of Optometry, Indian University, Bloomington, Indiana, United States
  • Chia-Yang Liu
    School of Optometry, Indian University, Bloomington, Indiana, United States
  • Footnotes
    Commercial Relationships   Yuka Okada, None; Yujin Zhang, None; Lingling Zhang, None; Yen-Chiao Wang, None; Chia-Yang Liu, None
  • Footnotes
    Support  NIH RO1 EY21501 EY 23086
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 1424. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Yuka Okada, Yujin Zhang, Lingling Zhang, Yen-Chiao Wang, Chia-Yang Liu; Shp2 protein tyrosine phosphatase affects corneal epithelial stratification and innervation. Invest. Ophthalmol. Vis. Sci. 2017;58(8):1424.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Corneal nerves are important for the detection of noxious stimuli at the surface of the cornea and for protection against corneal injury. We examine whether genetically impaired corneal epithelial stratification due to Shp2 deficiency or over expression can impact corneal innervation during development and nerve integrity in mature cornea.

Methods : Shp2 was conditionally knocked-out (Shp2cko) or ectopic expression of a murine gain-of-function mutant (Shp2cGOF) in the corneal epithelium administrated with doxycycline (Dox) from postnatal day 21 (P21) to P35, P50, and P110, respectively. Mouse eyes were subjected to histology and immunohistochemistry. In corneal epithelial wound healing experiment of Shp2cko, administrated with Dox from P60 to P74 were used. Time dependent closure of a 2.0 mm diameter central corneal epithelial debridement was monitored in the right eyes of mice for up to 48 h using fluorescein green staining.

Results : Shp2cko mice, corneal epithelial cell layers are decreased. Whole-mount examination of nerve specific protein, anti-neuron-specific β-tubulin antibody (TUJ1) positive nerve was dramatically reduced in Shp2cko mice as compare to control cornea. Conversely, corneal epithelial cell layers and TUJ1 positive nerve was increased in Shp2cGOF. In corneal wound healing model, the corneal epithelial defect remaining was larger in Shp2cko mice as compared with control mice.

Conclusions : These data argue that the corneal epithelial stratification is indispensable for corneal innervation. The Shp2cKO and Shp2cGOF mouse strains are potential models to study the role of epithelium-nerve interaction and also a novel animal models for neurotrophic keratopathy.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×