June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
The effect of coefficient of cell size variation on determination of endothelial cell density using multiple specular microscopy images
Author Affiliations & Notes
  • Jianyan Huang
    Image Reading Center, Doheny Eye Institute, Temple City, California, United States
    Department of Ophthalmology, David Geffen medical school, University of California, Los Angeles, Los Angeles, California, United States
  • Elmira Baghdasaryan
    Image Reading Center, Doheny Eye Institute, Temple City, California, United States
    Department of Ophthalmology, David Geffen medical school, University of California, Los Angeles, Los Angeles, California, United States
  • Ping Huang
    Image Reading Center, Doheny Eye Institute, Temple City, California, United States
    Department of Ophthalmology, David Geffen medical school, University of California, Los Angeles, Los Angeles, California, United States
  • Tudor Tepelus
    Image Reading Center, Doheny Eye Institute, Temple City, California, United States
    Department of Ophthalmology, David Geffen medical school, University of California, Los Angeles, Los Angeles, California, United States
  • Srinivas R Sadda
    Image Reading Center, Doheny Eye Institute, Temple City, California, United States
    Department of Ophthalmology, David Geffen medical school, University of California, Los Angeles, Los Angeles, California, United States
  • Olivia L Lee
    Image Reading Center, Doheny Eye Institute, Temple City, California, United States
    Department of Ophthalmology, David Geffen medical school, University of California, Los Angeles, Los Angeles, California, United States
  • Footnotes
    Commercial Relationships   Jianyan Huang, None; Elmira Baghdasaryan, None; Ping Huang, None; Tudor Tepelus, None; Srinivas Sadda, Allergan (C), Carl Zeiss Meditec (F), Centervue (C), Genentech (C), Iconic (C), Optos (C), Optos (F), Thrombogenics (C), Topcon (R); Olivia Lee, Allegan (C), Allegan (F)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 1459. doi:
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    • Get Citation

      Jianyan Huang, Elmira Baghdasaryan, Ping Huang, Tudor Tepelus, Srinivas R Sadda, Olivia L Lee; The effect of coefficient of cell size variation on determination of endothelial cell density using multiple specular microscopy images
      . Invest. Ophthalmol. Vis. Sci. 2017;58(8):1459.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Our previous study showed that a single best image could provide a representative value of the endothelial cell density (ECD) of the central cornea. The purpose of this study is to evaluate the effect of cell size variation on cell density (CD) variability across non-overlapping endothelial specular microscopy images in the central cornea when multiple images of equal image quality are available.

Methods : One hundred and eighty eyes without visible cornea pathologies were enrolled in this study. Sets of 3 images per eye were obtained with Konan NSP-9900 specular microscope (Konan Medical USA, Inc., Irvine, CA) and analyzed by 2 masked graders. Image quality rating (IQR) was graded based on a standardized IQR scale, while ECD and the coefficient of cell size variation (CV) were determined using the well-established Konan Center method. Only sets where 3 images were all scored to be of “good” quality were included in the study. Variations of ECD values from the 3 images were calculated. The correlation between the CD variation and CV cutoff values (CV ≥ 36; CV ≥ 38; CV ≥ 40; CV ≥ 45) were analyzed.

Results : A total of 173 eyes met the inclusion criteria and had 3 images rated to be of good quality. Inter-grader CD variation across the entire set was 2.4%±2.3%. For 109 eyes with CV ≤ 35, the mean CD variation was 2.3%±2.4%. When CV ≥ 36, the mean CD variation was 2.4%±2.1% (n=64). For CV ≥ 38, the mean CD variation was 2.3%±2.0% (n=34). When CV ≥ 40, the mean CD variation is 2.2%±2.3% (n=20). When CV ≥ 45, the mean CD variation was 3.2%±3.3% (n=6). However, regardless of degree of variation in cell size, there was no correlation between the ECD variation and the CV value (P>0.05).

Conclusions : CV does not appear to be related to the discrepancy in ECD values in the central cornea. Analysis of a best single image can provide a representative ECD value for the central cornea, even in the presence of polymegathism.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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