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Xuyang Liu, Junkai Tan, Yun Wang, Ning Fan, Paul L Kaufman, Ningli Wang; Exoenzyme C3 transferaselowered IOP in rats. Invest. Ophthalmol. Vis. Sci. 2017;58(8):1601.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the IOP-lowering effect of C3 exoenzyme (C3) using lentiviral vectors mediated C3 transduction in rat eyes.
Lentiviral vectors containing C3 (LV/C3) was prepared. Human trabecular meshwork (HTM) cells in culture were treated with LV/C3 at different MOI, and changes in cell morphology were observed. Five μl LV/C3 (8×108 TU/ml) or eight μl LV (5×108 TU/ml) were injected into the anterior chambers of the right and left eyes, respectively. The expression of GFP was visualized by Micron III Retinal Imaging Microscope and fluorescence microscope. IOP was measured every days post-injection for 8 days, and then every two days thereafter.
LV mediated C3 expression induced changes in HTM cell morphology. The GFP expression in the regions of the anterior chamber angle was observed by frozen-section examination in 8th days after injection of LV/C3 or LV. In the LV/C3 group, IOP was significantly decreased in 3rd day post-administration when compared to control eyes or baseline, and this effect lasted for at least40days (p < 0.05). No obvious inflammatory reactions were observed.
LV mediated C3 expression induced changes in cell morphology in cultured HTM cells, and lower the IOP when transduced into the anterior chamber of the rats.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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