June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Exoenzyme C3 transferaselowered IOP in rats
Author Affiliations & Notes
  • Xuyang Liu
    Shenzhen Eye Hospital, Shenzhen, Guangdong, China
  • Junkai Tan
    Shenzhen Eye Hospital, Shenzhen, Guangdong, China
  • Yun Wang
    Shenzhen Eye Hospital, Shenzhen, Guangdong, China
  • Ning Fan
    Shenzhen Eye Hospital, Shenzhen, Guangdong, China
  • Paul L Kaufman
    Department of Ophthalmology & Visual Science, University of Wisconsin-Madison, Madison, Wisconsin, United States
  • Ningli Wang
    Tongren Hospital, the Capital University of Medicine, Beijing, China
  • Footnotes
    Commercial Relationships   Xuyang Liu, None; Junkai Tan, None; Yun Wang, None; Ning Fan, None; Paul Kaufman, None; Ningli Wang, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 1601. doi:
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    • Get Citation

      Xuyang Liu, Junkai Tan, Yun Wang, Ning Fan, Paul L Kaufman, Ningli Wang; Exoenzyme C3 transferaselowered IOP in rats. Invest. Ophthalmol. Vis. Sci. 2017;58(8):1601.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To evaluate the IOP-lowering effect of C3 exoenzyme (C3) using lentiviral vectors mediated C3 transduction in rat eyes.

Methods : Lentiviral vectors containing C3 (LV/C3) was prepared. Human trabecular meshwork (HTM) cells in culture were treated with LV/C3 at different MOI, and changes in cell morphology were observed. Five μl LV/C3 (8×108 TU/ml) or eight μl LV (5×108 TU/ml) were injected into the anterior chambers of the right and left eyes, respectively. The expression of GFP was visualized by Micron III Retinal Imaging Microscope and fluorescence microscope. IOP was measured every days post-injection for 8 days, and then every two days thereafter.

Results : LV mediated C3 expression induced changes in HTM cell morphology. The GFP expression in the regions of the anterior chamber angle was observed by frozen-section examination in 8th days after injection of LV/C3 or LV. In the LV/C3 group, IOP was significantly decreased in 3rd day post-administration when compared to control eyes or baseline, and this effect lasted for at least40days (p < 0.05). No obvious inflammatory reactions were observed.

Conclusions : LV mediated C3 expression induced changes in cell morphology in cultured HTM cells, and lower the IOP when transduced into the anterior chamber of the rats.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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