June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Pre-clinical Model of Retinoblastoma Vitreous Seeds
Author Affiliations & Notes
  • Andrew S Irvine
    Ophthalmology, Hamilton Eye Institute, UTHSC, Memphis, Tennessee, United States
  • Zachary K. Goldsmith
    Ophthalmology, Hamilton Eye Institute, UTHSC, Memphis, Tennessee, United States
  • Rachel Brennan
    Ophthalmology, Hamilton Eye Institute, UTHSC, Memphis, Tennessee, United States
    Oncology, St. Jude Childrens Research Hospital, Memphis, Tennessee, United States
  • Matthew W Wilson
    Ophthalmology, Hamilton Eye Institute, UTHSC, Memphis, Tennessee, United States
    Surgery, St. Jude Childrens Research Hospital, Memphis, Tennessee, United States
  • Vanessa Marie Morales
    Ophthalmology, Hamilton Eye Institute, UTHSC, Memphis, Tennessee, United States
    Microbiology, Immunology and Biochemistry, UTHSC, Memphis, Tennessee, United States
  • Footnotes
    Commercial Relationships   Andrew Irvine, None; Zachary Goldsmith, None; Rachel Brennan, None; Matthew Wilson, None; Vanessa Morales, None
  • Footnotes
    Support  Research to Prevent Blindness
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 1770. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Andrew S Irvine, Zachary K. Goldsmith, Rachel Brennan, Matthew W Wilson, Vanessa Marie Morales; Pre-clinical Model of Retinoblastoma Vitreous Seeds
      . Invest. Ophthalmol. Vis. Sci. 2017;58(8):1770.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : The biggest challenge in treatment of retinoblastoma (Rb) is the presence of vitreous seeds. Vitreous seeding has a poorer prognosis for ocular salvage as reflected by multiple Rb classification systems. There is a lack of in vitro models of Rb vitreous seeds. Here, we adapt our in vitro 3D culture system to recreate Rb seeds in a vitreous-like microenvironment for more in-depth study.

Methods : Monocultures and co-cultures of CFSE-fluorescently labeled Y79 cells and human primary retinal microvascular endothelial cells (hRECs) were 3D cultured using magnetic levitation to re-create Rb seeds in the vitreous. We were able to visualize the growth of seeds over time in vitro by confocal microscopy.

Results : We generated in vitro Rb seeds similar to those found in Rb patients. The cultures were kept for over 2-weeks. Moreover, we were able to perform histological assessment to compare the 3D generated and ex vivo isolated seeds.

Conclusions : We have generated a pre-clinical model to investigate Rb seeds in vitro by using magnetic 3D cultures. Our novel methodology of fluorescently labeling the cells prior to magnetization allows isolation of both cell types to further characterize cells within the Rb microenvironment.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×