June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Evaluation of somatic mutations of KRAS, NRAS, and BRAF in Japanese patients with lacrimal gland epithelial tumors
Author Affiliations & Notes
  • Jun Ominato
    Division of Ophthalmology and Visual Science, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan
  • Tokuhide Oyama
    Division of Ophthalmology and Visual Science, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan
  • Hiroyuki Cho
    Division of Ophthalmology and Visual Science, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan
  • Takeo Fukuchi
    Division of Ophthalmology and Visual Science, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan
  • Ayako Sato
    Division of Molecular and Diagnostic Pathology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan
  • Yoichi Ajioka
    Division of Molecular and Diagnostic Pathology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan
  • Footnotes
    Commercial Relationships   Jun Ominato, None; Tokuhide Oyama, None; Hiroyuki Cho, None; Takeo Fukuchi, None; Ayako Sato, None; Yoichi Ajioka, None
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 1790. doi:
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    • Get Citation

      Jun Ominato, Tokuhide Oyama, Hiroyuki Cho, Takeo Fukuchi, Ayako Sato, Yoichi Ajioka; Evaluation of somatic mutations of KRAS, NRAS, and BRAF in Japanese patients with lacrimal gland epithelial tumors
      . Invest. Ophthalmol. Vis. Sci. 2017;58(8):1790.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To examine if somatic mutations of KRAS, NRAS, and BRAF are associated with lacrimal gland epithelial tumors in Japanese patients.

Methods : In this study, 7 cases of lacrimal gland epithelial tumors from a single institution were included. There were five cases of pleomorphic adenoma (PA), two cases of adenoid cystic carcinoma (ACC). DNA was extracted from formalin-fixed paraffin-embedded specimens. We amplified the DNA of KRAS exon 2, NRAS exon2, and BRAF exon 15 using PCR and examined each sample for mutation by direct sequencing.

Results : There was no KRAS, NRAS, or BRAF mutation in any of the cases. We also evaluated KRAS/NRAS exon2 mutation in the epithelial and mesenchymal components of PA, but no mutations were present in either component.

Conclusions : KRAS/NRAS exon2 and BRAF exon15 mutations were not found in 7 Japanese cases of lacrimal gland epithelial tumors. According to previous reports, no KRAS mutations have been observed in salivary gland ACC, and no BRAF mutations have been observed in other salivary gland tumors. This suggests that the genetic profile of lacrimal gland epithelial tumors may be similar to that of salivary gland tumors.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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