June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Detection of herpesviridae and presence of multiple co-infections in lymphoproliferative disorders of the ocular adnexa by comprehensive polymerase chain reaction assay
Author Affiliations & Notes
  • Yoshihiko Usui
    Ophthalmology, Tokyo Medical Univ Hospital, Shinjuku-ku, Japan
  • Sunao Sugita
    Laboratory for Retinal Regeneration, RIKEN Center for Developmental Biology, Kobe, Japan
  • Hiroshi Takase
    Tokyo Medical and Dental University, Tokyo, Japan
  • Shunichiro Ueda
    Ophthalmology, Tokyo Medical Univ Hospital, Shinjuku-ku, Japan
  • Kazuhiko Umazume
    Ophthalmology, Tokyo Medical Univ Hospital, Shinjuku-ku, Japan
  • Takeshi Kezuka
    Ophthalmology, Tokyo Medical Univ Hospital, Shinjuku-ku, Japan
  • Hiroshi Goto
    Ophthalmology, Tokyo Medical Univ Hospital, Shinjuku-ku, Japan
  • Footnotes
    Commercial Relationships   Yoshihiko Usui, None; Sunao Sugita, None; Hiroshi Takase, None; Shunichiro Ueda, None; Kazuhiko Umazume, None; Takeshi Kezuka, None; Hiroshi Goto, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 1798. doi:
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    • Get Citation

      Yoshihiko Usui, Sunao Sugita, Hiroshi Takase, Shunichiro Ueda, Kazuhiko Umazume, Takeshi Kezuka, Hiroshi Goto; Detection of herpesviridae and presence of multiple co-infections in lymphoproliferative disorders of the ocular adnexa by comprehensive polymerase chain reaction assay
      . Invest. Ophthalmol. Vis. Sci. 2017;58(8):1798.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Infectious agents have been identified as a major cause of specific types of human cancers worldwide. Several microorganisms have been identified as potential aggravators of ocular adnexal neoplasms. To conduct an exhaustive search for pathogenic DNA in lymphoproliferative disorders (LPD) of the ocular adnexa.

Methods : From 2008 to 2013, a total of 70 patients diagnosed with LPD of the ocular adnexa were studied. Specimens were screened for bacterial, viral, fungal, and parasitic DNA by multiplex polymerase chain reaction (PCR) and quantitative real-time PCR. Copy number values of more than 50 copies/μg DNA (virus and parasite) or 100 copies/μg DNA (bacteria and fungi) in the biopsy sample were considered to be significant.

Results : Among 19 cases of conjunctival mucosa-associated lymphoid tissue (MALT) lymphoma, human herpes virus (HHV)-6 DNA was detected in 1 case, HHV-7 DNA in 1 case, chlamydia DNA in 1 case, and Epstein-Barr virus (EBV) DNA in 3 cases. HHV-6 DNA was detected in only one case of orbital MALT lymphoma. Among 22 cases of IgG4-related ocular disease, HHV-6 DNA was detected in 5 cases, HHV-7 DNA in 8 cases, and EBV-DNA in 7 cases. Multiple infections were detected in 7 of 22 cases. In 7 cases of orbital reactive lymphoid hyperplasia, HHV-6 DNA was detected in 2 cases, HHV-7 DNA in 3 cases, and EBV-DNA in 2 cases.

Conclusions : Our comprehensive PCR assays detected various and multiple infectious antigen DNAs in tumor specimens, especially herpes viruses HHV6, HHV7, and EBV. Chronic stimulation or oncogenes from these infectious agents might be involved in the pathogenesis of LPD of the ocular adnexa.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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