June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Gene Expression Profile of Trabecular Meshwork from Primary Open-Angle Glaucoma Patient by RNA-Sequencing
Author Affiliations & Notes
  • Mingzhi Zhang
    Ophthalmology, Joint Shantou International Eye Center, Shantou, China
  • Footnotes
    Commercial Relationships   Mingzhi Zhang, None
  • Footnotes
    Support  NSFC: Grant 81486126
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 2141. doi:
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    • Get Citation

      Mingzhi Zhang; Gene Expression Profile of Trabecular Meshwork from Primary Open-Angle Glaucoma Patient by RNA-Sequencing. Invest. Ophthalmol. Vis. Sci. 2017;58(8):2141.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The purpose of this study is to investigate gene expression profile in trabecular meshwork from POAG patient or control, and to analyze whether the function of the genes with different expression.
Methods: POAG trabecular meshwork specimen was obtained from trabeculectomy surgery. Non-glaucomatous control trabecular meshwork specimen was dissected from donor eye. RNA was extracted and sequenced; we got the gene expression profiles and analyzed whether the gene expression differences associate with POAG.

Methods : POAG trabecular meshwork specimen was obtained from trabeculectomy surgery. Non-glaucomatous control trabecular meshwork specimen was dissected from donor eye. RNA was extracted and sequenced; we got the gene expression profiles and analyzed whether the gene expression differences associate with POAG.

Results : Totally 11567 genes were got, 3901 significantly altered genes were detected across the genome, 426 genes were up-regulated and 3475 genes were down-regulated. Gene ontology (GO) enrichment of differentially expressed genes involved catalytic activity, signaling pathway, metabolic process, biological regulation, developmental process. Genes involved in development process of ectoderm were mostly down-regulated; Matrix metallopeptidase 1 and genes related to extracellular matrix were mostly down-regulated; Carbonic anhydrase III related to regulate aqueous humor were mostly up-regulated.

Conclusions : The change of gene expression profile of trabecular meshwork from primary open-angle glaucoma patient may be associated with structure change and increase formation of aqueous humor.
Key words: Primary open-angle glaucoma, trabecular meshwork, RNA-sequencing.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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