June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Developmental endocrine disruption alters adult visually-guided behaviors in the Zebrafish.
Author Affiliations & Notes
  • Cassie Janell Gould
    Biology, American University, Washington, District of Columbia, United States
  • Colin J Saldanha
    Biology, American University, Washington, District of Columbia, United States
  • Victoria P Connaughton
    Biology, American University, Washington, District of Columbia, United States
  • Footnotes
    Commercial Relationships   Cassie Gould, None; Colin Saldanha, None; Victoria Connaughton, None
  • Footnotes
    Support   Grant in Aid of Research from Sigma Xi, The Scientific Research Society G201510151622928 (CJG); American University Faculty Mellon Grant (VPC); American University College of Arts & Sciences Graduate Student Support Competition, Fall 2015, Spring 2016 (CJG)
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 2226. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Cassie Janell Gould, Colin J Saldanha, Victoria P Connaughton; Developmental endocrine disruption alters adult visually-guided behaviors in the Zebrafish.. Invest. Ophthalmol. Vis. Sci. 2017;58(8):2226.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To determine the short- and long-term effects of two anti-estrogenic endocrine disruptors, formestane and PCB1254, on larval visual system anatomy and adult visually-guided behaviors.

Methods : Zebrafish larvae were transiently exposed to environmentally-relevant concentrations of each chemical at 24hpf (hours postfertilization), 72hpf, 7d (days) pf, and 21dpf and the acute effects on overall anatomy were determined. At 3-months of age, adult optomotor responses were assessed. To determine the mechanism of action of the endocrine disruptors, aromatase activity was measured using adult zebrafish brain tissue.

Results : There were no significant anatomical differences nor an effect on aromatase activity (One Way ANOVA, p=0.86) as a result of acute exposure to PCB1254. In contrast, acute development exposure to formestane potently inhibited aromatase activity (One way ANOVA, p<0.01) and significantly diminished adult visually guided behaviors, as there was a main effect of treatment between the control stimulus ON and treatment stimulus ON groups (2x2x2 ANOVA, p<0.01). Interestingly, however, there were no significant gross morphological differences as a result of formestane exposure.

Conclusions : Inhibition of aromatase activity by formestane during specific developmental time points results in diminished visually-guided responses in adults suggesting long-term consequences.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×