June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Protective effect of persimmon leaves (Diospyros kaki) on dry eye in an exorbital lacrimal gland excision model of mice.
Author Affiliations & Notes
  • CHUNGHYUN LEE
    Ophthalmology, University of Ulsan, College of Medicine, Gangneung Asan Hospital, Gangneung, Korea (the Republic of)
  • Kyung-A Kim
    Natural Products Research Center, Korea Institute of Science and Technology (KIST), Gangneung, Korea (the Republic of)
    Biological Chemistry, University of Science and Technology (UST), Daejeon, Korea (the Republic of)
  • SUNGJAE YANG
    Ophthalmology, University of Ulsan, College of Medicine, Gangneung Asan Hospital, Gangneung, Korea (the Republic of)
    Ophthalmology (Casey Eye Ins.), Oregon Health & Science University, Portland, Oregon, United States
  • SANGHOON JUNG
    Natural Products Research Center, Korea Institute of Science and Technology (KIST), Gangneung, Korea (the Republic of)
    Biological Chemistry, University of Science and Technology (UST), Daejeon, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   CHUNGHYUN LEE, None; Kyung-A Kim, None; SUNGJAE YANG, None; SANGHOON JUNG, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 2252. doi:
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      CHUNGHYUN LEE, Kyung-A Kim, SUNGJAE YANG, SANGHOON JUNG; Protective effect of persimmon leaves (Diospyros kaki) on dry eye in an exorbital lacrimal gland excision model of mice.. Invest. Ophthalmol. Vis. Sci. 2017;58(8):2252.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To evaluate the effects of ethanol extract of Diospyros kaki (EEDK) on dry eye in an exorbital lacrimal gland excision model of mice.

Methods : Unilateral excision of the exorbital lacrimal gland was performed on BALB/C mice. In order to the effectiveness of the EEDK, it was orally administered throughout the experimental period. We performed tear break-up time (BUT) test, fluorescein staining, and histological analysis on the ocular surface after surgery. The protein expression levels of Mucin 5AC (MUC5AC) and apoptosis-associated proteins (Bax, cytochrome c, and caspase-3) detected by western blotting. Moreover, apoptotic cell death was tested by in situ terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The squamous epithelium cells were evaluated by the immunohistochemistry (IHC).

Results : Oral administration of EEDK resulted in prolonged tear break-up time (BUT), decreased fluorescein score, and smoother epithelial cells compared with lacrimal gland excision alone in the cornea. Moreover, down-regulated MUC5AC, up-regulated apoptotic-associated proteins, elevated squamous epithelium cells, and increased apoptotic cells (TUNEL positive cells) were inhibited by treatment with EEDK.

Conclusions : EEDK has potential as a beneficial agent in the clinical treatment of dry eye.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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