June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Synergistic attenuation of choroidal neovascularization by dietary intake of ω-3 long-chain polyunsaturated fatty acids and lutein in mice
Author Affiliations & Notes
  • Ryoji Yanai
    Ophthalmology, Yamaguchi Univ Grad Sch of Med, Ube City, Japan
  • Shang Chen
    Ophthalmology, Yamaguchi Univ Grad Sch of Med, Ube City, Japan
  • Sho-hei Uchi
    Ophthalmology, Yamaguchi Univ Grad Sch of Med, Ube City, Japan
  • Tomoaki Nanri
    SANTEN PHARMACEUTICAL CO., LTD., Osaka, Japan
  • Kazuhiro Kimura
    Ophthalmology, Yamaguchi Univ Grad Sch of Med, Ube City, Japan
  • Footnotes
    Commercial Relationships   Ryoji Yanai, Sanren Phrmacological Co. ltd (F); Shang Chen, None; Sho-hei Uchi, None; Tomoaki Nanri, Sanren Phrmacological Co. ltd (E); Kazuhiro Kimura, Sanren Phrmacological Co. ltd (F)
  • Footnotes
    Support  A grant (15K10869) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 2304. doi:
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      Ryoji Yanai, Shang Chen, Sho-hei Uchi, Tomoaki Nanri, Kazuhiro Kimura; Synergistic attenuation of choroidal neovascularization by dietary intake of ω-3 long-chain polyunsaturated fatty acids and lutein in mice. Invest. Ophthalmol. Vis. Sci. 2017;58(8):2304.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Dietary ω-3 long-chain polyunsaturated fatty acids (LCPUFAs) have been found to protect against age-related macular degeneration (AMD), the primary cause of blindness in elderly individuals of industrialized countries. Lutein is a major carotenoid in the macula and retina, where it protects against the adverse effects of photochemical reactions as a result of its antioxidant properties. Although lutein also protects against AMD, its effects in combination with ω-3 LCPUFAs have remained unknown. We therefore examined the effects of dietary intake of both ω-3 LCPUFAs and lutein in a mouse model of AMD.

Methods : C57BL/6 mice were fed a diet enriched in or free of ω-3 LCPUFAs beginning 2 weeks before, and either containing lutein (2.5 mg/kg per day) or not beginning 1 week before, induction of choroidal neovascularization (CNV) by laser photocoagulation. The area of CNV was evaluated in choroidal flat-mount preparations 7 days after photocoagulation. Primary eicosanoids and their metabolites were analyzed by liquid chromatography and tandem mass spectrometry. Expression of cytokines and chemokines in the retina or choroid was examined by RT-PCR analysis and with the use of a multiplex assay system, and that of NADPH oxidase 4 (Nox4) by immunoblot analysis.

Results : The area of CNV was significantly reduced in mice fed ω-3 LCPUFAs or lutein compared with the control diet, and it was synergistically reduced in those fed both ω-3 LCPUFAs and lutein. The serum concentrations of the principal ω-3 LCPUFAs and lutein were significantly increased in mice fed the corresponding diets. The expression of cytokines and chemokines was reduced at the mRNA and protein levels in the retina or choroid of ω-3 LCPUFA–fed mice, lutein-fed mice, and mice fed both dietary components, but no synergistic effect was apparent. The abundance of Nox4 in the retina was significantly reduced in mice fed ω-3 LCPUFAs and synergistically reduced in those receiving both ω-3 LCPUFAs and lutein.

Conclusions : Dietary intake of ω-3 LCPUFAs and lutein attenuated the development of CNV in a synergistic manner, with this effect being accompanied by down-regulation of Nox4, a major source of reactive oxygen species in the retina. Dietary supplementation with both ω-3 LCPUFAs and lutein warrants further study as a means to protect against AMD.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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