June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
A2E-blue light induced endothelial invasion by using 3-deimensional choroidal neovascularization model
Author Affiliations & Notes
  • Kyoung-pil Lee
    Kyungpook National University, School of medicine, Daegu, Korea (the Republic of)
  • Man-Il Huh
    Kyungpook National University, School of medicine, Daegu, Korea (the Republic of)
  • Jeongho Kim
    Kyungpook National University, School of medicine, Daegu, Korea (the Republic of)
  • Byeong-Ung Park
    Kyungpook National University, School of medicine, Daegu, Korea (the Republic of)
  • Soo-Jin Yi
    Kyungpook National University, School of medicine, Daegu, Korea (the Republic of)
  • Myung-Jun Kim
    Kyungpook National University, School of medicine, Daegu, Korea (the Republic of)
  • Hong Kyun Kim
    Kyungpook National University, School of medicine, Daegu, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   Kyoung-pil Lee, None; Man-Il Huh, None; Jeongho Kim, None; Byeong-Ung Park, None; Soo-Jin Yi, None; Myung-Jun Kim, None; Hong Kyun Kim, None
  • Footnotes
    Support  This study was supported by Korean National Research Fund (NRF-2012R1A1A1010163) and the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI15C0001)
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 2312. doi:
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      Kyoung-pil Lee, Man-Il Huh, Jeongho Kim, Byeong-Ung Park, Soo-Jin Yi, Myung-Jun Kim, Hong Kyun Kim; A2E-blue light induced endothelial invasion by using 3-deimensional choroidal neovascularization model. Invest. Ophthalmol. Vis. Sci. 2017;58(8):2312.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Accumulation of A2E and Exposure of blue light are a major factor of marcular degeneration. These are related with angiogenesis between retinal pigment epithelial (RPE) cells and choroid. There were no 3-deimensional (D) choroidal neovascularization (CNV) model to describe both accumulation of A2E, optical stimulus and movement of endothelial cells. We developed 3D retinal-vascular mimetic system reflecting angiogenesis induced by A2E-blue light damage.

Methods : Human RPE cells and peripheral micro-vascular endothelial (PMVE) cells were co-cultured in a microfluidic channel. hRPEs were pretreated with 10 µM A2E, and then the cells were exposed with/without blue light (the channel of RPE cells were exposed to 400 ± 20 nm for 30 seconds). A hypoxic condition for 24hrs was used as positive control of CNV. We examined the chemical and optical stimulus on 3D retinal-vascular mimetics system, and then evaluated cell invasion, Vascular endothelial growth factor (VEGF) secretion and activation of the signal mediators.

Results : In the 3D retinal-vascular mimetic system between RPE and PMVE cells, PMVE cell were invaded into RPE cells in hypoxia (21.0 ± 3.93 cell/unit area), A2E (22.3 ± 4.26 cell/unit area), and A2E with blue light (36.3 ± 5.02 cell/unit area) conditions for 24hrs. In the addition, Secretion of VEGF by RPE in the 3D retinal-vascular mimetics system increased A2E with/without blue light and hypoxia conditions by ELISA assay for 24 hrs.

Conclusions : In this study, data indicated A2E-blue light induced the secretion of VEGF and the invasion of PMVE cells in 3D co-culture model. We established new 3D retinal-vascular mimetic model, an angiogenesis model related with CNV, would be able to detect invasion of endothelial cells under a disease condition. This model would be to employ for a candidate selection before a pre-clinical trial.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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