June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Monocytes from patients with diabetic retinopathy are characterized by increased VEGF transcription during early macrophage differentiation
Author Affiliations & Notes
  • Xavier Guillonneau
    Institut de la Vision, UPMC UMRS968, Paris, France
  • David Rivera
    Institute of Ophthalmology, Conde de Valenciana Foundation, Mexico city, Mexico
    Centro de Atención Integral Del Paciente Con Diabetes, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico city, Mexico
  • Hugo Charles-Messance
    Institut de la Vision, UPMC UMRS968, Paris, France
  • Aida Jimenez-Corona
    Institute of Ophthalmology, Conde de Valenciana Foundation, Mexico city, Mexico
  • Aude COUTURIER
    Institut de la Vision, UPMC UMRS968, Paris, France
  • Alvaro Rendon
    Institut de la Vision, UPMC UMRS968, Paris, France
  • Jose Alain Sahel
    Institut de la Vision, UPMC UMRS968, Paris, France
  • Chiara M Eandi
    Department of Clinical Science, Eye Clinic, University of Torino, Torino, Italy
  • Florian Sennlaub
    Institut de la Vision, UPMC UMRS968, Paris, France
  • Enrique O Graue-Hernandez
    Institute of Ophthalmology, Conde de Valenciana Foundation, Mexico city, Mexico
  • Yonathan Garfias
    Institute of Ophthalmology, Conde de Valenciana Foundation, Mexico city, Mexico
    Department of Biochemistry, Universidad Nacional Autónoma de México,Faculty of Medicine, Mexico city, Mexico
  • Footnotes
    Commercial Relationships   Xavier Guillonneau, None; David Rivera, None; Hugo Charles-Messance, None; Aida Jimenez-Corona, None; Aude COUTURIER, None; Alvaro Rendon, None; Jose Sahel, None; Chiara Eandi, None; Florian Sennlaub, None; Enrique Graue-Hernandez, None; Yonathan Garfias, None
  • Footnotes
    Support  Instituto de Oftalmologia Conde de Valenciana Foundation, FIAPSIL
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 2529. doi:
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      Xavier Guillonneau, David Rivera, Hugo Charles-Messance, Aida Jimenez-Corona, Aude COUTURIER, Alvaro Rendon, Jose Alain Sahel, Chiara M Eandi, Florian Sennlaub, Enrique O Graue-Hernandez, Yonathan Garfias; Monocytes from patients with diabetic retinopathy are characterized by increased VEGF transcription during early macrophage differentiation. Invest. Ophthalmol. Vis. Sci. 2017;58(8):2529.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Type 2 diabetes (T2DM) affects a growing part of the population in all developed countries. More than 60% of people with T2DM will develop a form a Diabetic Retinopathy (DR) after 20 years of diabetes. Diabetic patients demonstrate robustly elevated intravitreal levels of inflammatory cytokines. Infiltrating monocyte (Mo)-derived inflammatory Macrophages (MPs) account for most of the production of these cytokines in animal models of DR. We hypothesized that pre-activated circulating inflammatory monocytes found in T2DM are an important pathogenic factor in DR.

Methods : T2DM and control patients were enrolled and diagnosed for diabetic retinopathy. Four groups were defined as follows: (i) controls, (ii) diabetic patients with no retinopathy, (iii) patients with NPDR, and (iv) PDR patients. Patients with known history of nephropathy and periodontitis were excluded. Plasma and monocytes were collected from all groups. Fresh monocytes were allowed to differentiate into MPs for 18h and RNA and culture supernatant were collected. Fresh Monocytes and monocyte-derived MPs were then evaluated for their ability to produce cytokines known to be elevated in the vitreous of DR patients including IL-1β, IL-6, IL-8, CCL2, and VEGF

Results : Seventy-two subjects were included. The 3 diabetic groups were evenly distributed for age, BMI, blood glucose and Hba1c. Circulating monocytes demonstrated low expression of cytokines with minor differences between groups. In contrast, upon differentiation MP increased their expression of all tested cytokines. After 18h the expression of VEGF was higher in patients with NPDR or PDR than in diabetic patients with no retinopathy.

Conclusions : Our data support a model in which circulating monocytes in DR patients have a greater propensity to produce inflammatory cytokines when they differentiate into inflammatory macrophages compared to T2DM patients. Understanding how monocyte derived-inflammatory cytokine influence DR and treatment outcomes might be new cues to propose innovative treatments of these pathologies.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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