June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Post-ischemic treatment with azithromycin prevents retinal ganglion cell death induced by ischemia/reperfusion injury in rat
Author Affiliations & Notes
  • Annagrazia Adornetto
    Pharmacy and Health and Nutritional Sciences, University of Calabria, Arcavacata di Rende, Cosenza, Italy
  • Giuseppe Pasquale Varano
    Pharmacy and Health and Nutritional Sciences, University of Calabria, Arcavacata di Rende, Cosenza, Italy
  • Carlo Nucci
    Experimental Medicine and Surgery, Ophthalmology Unit, Rome, Italy
  • Maria Tiziana Corasaniti
    Health Sciences, University “Magna Graecia”, Catanzaro, Italy
  • Luigi Antonio Morrone
    Pharmacy and Health and Nutritional Sciences, University of Calabria, Arcavacata di Rende, Cosenza, Italy
  • Giacinto Bagetta
    Pharmacy and Health and Nutritional Sciences, University of Calabria, Arcavacata di Rende, Cosenza, Italy
  • Rossella Russo
    Pharmacy and Health and Nutritional Sciences, University of Calabria, Arcavacata di Rende, Cosenza, Italy
  • Footnotes
    Commercial Relationships   Annagrazia Adornetto, None; Giuseppe Varano, None; Carlo Nucci, None; Maria Corasaniti, None; Luigi Morrone, None; Giacinto Bagetta, None; Rossella Russo, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 2564. doi:
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    • Get Citation

      Annagrazia Adornetto, Giuseppe Pasquale Varano, Carlo Nucci, Maria Tiziana Corasaniti, Luigi Antonio Morrone, Giacinto Bagetta, Rossella Russo; Post-ischemic treatment with azithromycin prevents retinal ganglion cell death induced by ischemia/reperfusion injury in rat
      . Invest. Ophthalmol. Vis. Sci. 2017;58(8):2564.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Azithromycin is a widely used semi-synthetic macrolide antibiotic endowed with anti-inflammatory and immunomodulatory properties. Inflammation has been identified as an important component in the pathogenesis and progression of glaucoma and a potential target to achieve retinal neuroprotection. Here we studied the effect of azithromycin on retinal ganglion cell (RGC) degeneration induced by transient increase of intraocular pressure in rat.

Methods : Retinal ischemia was induced in adult rats by transient elevation of intraocular pressure. RGCs were retrogradely labeled by Fluorogold and survival was assessed following a single dose of azithromycin or vehicle given systemically at the end of the ischemia. Expression of death-associated proteins and ERK activation were studied by western blotting. Expression and activity of MMP-2 and -9 were analyzed by gelatin zymography.

Results : Acute post-injury administration of azithromycin significantly prevented RGC death. This effect was accompanied by reduced calpains activity and prevention of Bad upregulation. The observed neuroprotection was associated with a significant inhibition of MMP-2/-9 gelatinolytic activity and ERK1/2 phosphorylation.

Conclusions : Azithromycin affords neuroprotection by modifying the inflammatory state of the retina following ischemia/reperfusion injury suggesting its potential repurposing for glaucoma treatment.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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