June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Promoting optic nerve regeneration with a novel biocompatible fibrous scaffold
Author Affiliations & Notes
  • Karen Chang
    Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan
    Department of Ophthalmology, Schepens Eye Research Institute and Massachusetts Eye and Ear, Boston, Massachusetts, United States
  • Jhih-Guang Wu
    Department of Materials Science and Engineering, National Taiwan University, Taipei, Taiwan
  • Kin-Sang Cho
    Department of Ophthalmology, Schepens Eye Research Institute and Massachusetts Eye and Ear, Boston, Massachusetts, United States
  • Shyh-Chyang Luo
    Department of Materials Science and Engineering, National Taiwan University, Taipei, Taiwan
  • Ta-Ching Chen
    Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan
  • Min-Huey Chen
    Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan
  • Wei-Fang Su
    Department of Materials Science and Engineering, National Taiwan University, Taipei, Taiwan
  • Dong Feng Chen
    Department of Ophthalmology, Schepens Eye Research Institute and Massachusetts Eye and Ear, Boston, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Karen Chang, None; Jhih-Guang Wu, None; Kin-Sang Cho, None; Shyh-Chyang Luo, None; Ta-Ching Chen, None; Min-Huey Chen, None; Wei-Fang Su, None; Dong Chen, None
  • Footnotes
    Support  MOST (105-2917-I-002-031), NIH/NEI (R41 EY025913 and R01EY025259), the Core Grant for Vision Research from NIH/NEI to the Schepens Eye Research Institute (P30EY03790)
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 2592. doi:
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    • Get Citation

      Karen Chang, Jhih-Guang Wu, Kin-Sang Cho, Shyh-Chyang Luo, Ta-Ching Chen, Min-Huey Chen, Wei-Fang Su, Dong Feng Chen; Promoting optic nerve regeneration with a novel biocompatible fibrous scaffold. Invest. Ophthalmol. Vis. Sci. 2017;58(8):2592.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Glaucoma, a neurodegenerative disease, leads to progressive nerve damage and eventually loss of vision. Once the degeneration is occurred, it is permanent and irreversible. The purpose of this study is to develop a biocompatible scaffold for promoting retinal ganglion cells (RGC) survival and optic nerve regeneration after injury.

Methods : Polycaprolactone (PCL) and poly-gamma-benzyl-L-glutamate (PBG) fibrous scaffolds were prepared by 3D electrospinning with different fiber arrangements. To investigate the biocompatibility of those scaffolds, retinal ganglion cell progenitors (RGCP) were cultured on either aligned fibers or non-aligned fibers of PBG or PCL scaffolds for 1, 5, 10 days respectively. Cell survival, morphology, as well as neurite outgrowth rate and neurite length were observed by immunocytochemistry staining characterization.

Results : The biomimetic electrospun scaffolds for PCL and PBG featuring uniform fibrous structures and three dimensional porous networks. PBG scaffold with aligned fibers were successfully fabricated, supporting cell survival and robust growth of long neurite along the direction of the fibers.

Conclusions : The aligned PBG scaffold showed a great potential to assist RGCP differentiation and growth. Such scaffold may not only be beneficial for optic nerve regeneration, but also have a wide range of applications for tissue engineering on future regenerative medicine.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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