June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Effects of travoprost on ZO-1 expression in corneal epithelial cells in vitro
Author Affiliations & Notes
  • Yukihisa Takada
    Ophthalmology, Wakayama Medical University, Wakayama, Japan
  • Osamu Yamanaka
    Ophthalmology, Wakayama Medical University, Wakayama, Japan
  • Yuka Okada
    Ophthalmology, Wakayama Medical University, Wakayama, Japan
  • Takayoshi Sumioka
    Ophthalmology, Wakayama Medical University, Wakayama, Japan
  • Shizuya Saika
    Ophthalmology, Wakayama Medical University, Wakayama, Japan
  • Footnotes
    Commercial Relationships   Yukihisa Takada, None; Osamu Yamanaka, None; Yuka Okada, None; Takayoshi Sumioka, None; Shizuya Saika, None
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 2623. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Yukihisa Takada, Osamu Yamanaka, Yuka Okada, Takayoshi Sumioka, Shizuya Saika; Effects of travoprost on ZO-1 expression in corneal epithelial cells in vitro. Invest. Ophthalmol. Vis. Sci. 2017;58(8):2623.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To examine the effects of travoprost, an ingredient of prostaglandin-glaucoma eye drop, on ZO-1 expression in cultured corneal epithelial cells. We previously reported that travoprost upregulates expression of epidermal growth factor ( EGF ) and induced cell proliferation in cultured corneal epithelial cells and epithelium of an organ-cultured mouse cornea. Such effects of travoprost were cancelled by further addition of PD168393 ( PD ), an EGF receptor inhibitor ( ARVO2016 ).

Methods : Human corneal epithelial cells ( HCEC ) were cultured for 24 hrs in the presence or absence of travoprost ( 0.04 g/l ) and/or PD ( 10 μM ). Expression of ZO-1 was evaluated by using immunocytochemistry and western blotting. Mouse eyeball was organ-cultured for 48h in the presence or absence of travoprost and/or PD. We examined expression of zo-1 in HCEC and in an organ-cultured mouse eyes by using immunohistochemistry.

Results : Adding travoprost impaired cell-cell contact localization of ZO-1 and its protein expression in cultured cells. Further addition of PD canceled disturbance of normal localization of ZO-1, but did not reverse the suppression of its protein expression. In the organ-culture, adding travoprost reduced the expression level of ZO-1 in corneal epithelium and such suppression was reversed by further addition of PD.

Conclusions : Travoprost down-regulated ZO-1 cell-cell adhesion, and the effect of travoprost was canceled by further addition of PD in cultured corneal epithelial cells and epithelium of an organ-cultured mouse cornea. Upregulation of EGF by travoprost (ARVO 2016) could explain the mechanism of the current data of expression pattern modulation of ZO-1 with travoprost with or without PD. The findings might explain the additional mechanism of travoprost-related corneal epithelial disorders.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×