June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
The effect of rapamycin on autophagy in human corneal epithelial cells
Author Affiliations & Notes
  • Samaneh Ghasemi
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Xiang Shen
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Gaurav Agnihotri
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Ilham Putra
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Yasmin Rassouli
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Medi Eslani
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Ali R Djalilian
    Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois, United States
  • Footnotes
    Commercial Relationships   Samaneh Ghasemi, None; Xiang Shen, None; Gaurav Agnihotri, None; Ilham Putra, None; Yasmin Rassouli, None; Medi Eslani, None; Ali Djalilian, None
  • Footnotes
    Support  Clinical Scientist Development Program Award K12EY021475 (ME), R01 EY024349-01A1 (ARD) and Core grant EY01792 from NEI/NIH; MR130543 (ARD) from DoD, Vision for Tomorrow (ARD), unrestricted grant to the department from RPB; and Eversight (providing both seed funding and human corneal research tissue).
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 2627. doi:
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    • Get Citation

      Samaneh Ghasemi, Xiang Shen, Gaurav Agnihotri, Ilham Putra, Yasmin Rassouli, Medi Eslani, Ali R Djalilian; The effect of rapamycin on autophagy in human corneal epithelial cells. Invest. Ophthalmol. Vis. Sci. 2017;58(8):2627.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Rapamycin has been shown to have anti-aging effects in cells and animals. We have previously reported that rapamycin prolongs the survival of corneal epithelial cells in culture and prevents their loss to replicative senescence. In this study, we investigated whether rapamycin, at similar doses, increases autophagy in human corneal epithelial cells.

Methods : Human primary corneal epithelial cells were cultured in serum-free media. The cells were treated with rapamycin or DMSO (control). The cell morphology and the expression of autophagy marker LC3 by immunostaining and Western blot were noted.

Results : Rapamycin treatment of corneal epithelial cells induced the formation of vacuoles (autophagosomes) which were visible by light microscopy. The vacuoles were found to express LC3. Rapamycin likewise increased the expression of LC3 by Western blot.

Conclusions : Rapamycin appears to increase autophagy in primary human corneal epithelial cells. This may in part explain the increased survival of corneal epithelial cells in culture.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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