June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Nucleic acid stimulation increases the membrane-associated mucin expression in immortalized corneal and conjunctival epithelium
Author Affiliations & Notes
  • Yuriko Ban
    Nantan General Hospital, Nantan, Japan
    Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • Saho Morita
    Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Japan
  • Chie Sotozono
    Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • Shigeru Kinoshita
    Frontier Medical Science and Technology for Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
  • Footnotes
    Commercial Relationships   Yuriko Ban, None; Saho Morita, None; Chie Sotozono, None; Shigeru Kinoshita, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 2628. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Yuriko Ban, Saho Morita, Chie Sotozono, Shigeru Kinoshita; Nucleic acid stimulation increases the membrane-associated mucin expression in immortalized corneal and conjunctival epithelium. Invest. Ophthalmol. Vis. Sci. 2017;58(8):2628.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To investigate the response to nucleic acid stimulation in the membrane-associated mucin expression in immortalized corneal and conjunctival epithelium.

Methods : Immortalized human corneal epithelial cells (HCLE) and human conjunctival epithelial cells (HCjE) were cultured on 12-mm Transwell filters (n=12) at a density of 4x105 cells/cm2. The cultured cells were then stimulated with 25µg/ml of polyinosinic:polycytidylic acid [Poly(I:C)], an analog of viral double-stranded RNA produced during viral replication. Transepithelial electrical resistance (TER) was then measured using EndOhm electrodes (World Precision Instruments). After 6, 12, and 24 hours of exposure to Poly(I:C), the expressions of membrane-associated mucins (MUC1, MUC4, and MUC16) were analyzed by Western blotting. Immunoreactive bands were visualized by chemiluminescence, and densitometry analysis was then performed.

Results : Poly(I:C) challenge increased the TER in a time-dependent manner. After 6 hours and 12 hours of Poly(I:C) exposure, no change was observed in all 3 membrane-associated mucins. After 24 hours, membrane-associated mucins were increased.

Conclusions : The findings of this study show that Poly(I:C) challenge, which mimics a viral infection, increased the barrier function of ocular surface epithelia by increasing the membrane-associated mucin expression. Thus, we theorize that the increased barrier function must be a kind of host defense reaction to viral infection.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×