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catherine chia, Elizabeth Winters, Huyen Nguyen, Diego Tapias, Jeanette hyer, Jay M Stewart, Ricardo Lamy; In ovo chick chorioallantoic membrane (CAM) model to assess vascular reactivity.. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3042.
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© ARVO (1962-2015); The Authors (2016-present)
The chicken embryo chorioallantoic membrane (CAM) has been extensively used in many models forstudying angiogenesis and surgical procedures. The purpose of this study is to develop a rapid and low cost in vivomodel using CAM to assess vascular reactivity of eye drops and other topical drugs and drug candidates.
The in ovo CAM was prepared for experimentation without its inner shell membrane 48h before theapplication of the experimental drugs. A thin sterile silicone ring was applied on top of the CAM to demarcate thevascular area to be tested. Eye drops and topical drugs were applied inside the silicone ring and a series of pictureswere taken at 0, 2, 5, 10, 15 and 20 minutes using a cellphone camera adapted to a microscope. The diameter of thevessels was assessed at each time point and compared using the publicly available ImageJ software.
Changes in the vascular diameter were clearly observed and easily documented for image post processingand quantification. Vasodilation with a maximum increase of 106% on the vessel diameter was observed after 5minutes of application of topical propranolol 6mg/ml solution. Phenylephrine 2.5% eye drops promoted the maximumobserved vasoconstriction, with total closure of some vessels after 15 minutes.
This in ovo CAM model is a low cost, easily accessible, quantifiable, in vivo model to screen and studyvascular reactivity of eye drops and other topical drugs.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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