June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
The influence of hard and soft contact lenses on tear protein profiles: A perspective through the proteomic looking glass
Author Affiliations & Notes
  • Caroline Manicam
    Department of Ophthalmology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
  • Natarajan Perumal
    Department of Ophthalmology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
  • Yong Cajetan Ngongkole
    Department of Ophthalmology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
  • Alexandra Tschäbunin
    Department of Ophthalmology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
  • Marcel Sievers
    Department of Ophthalmology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
  • Franz H Grus
    Department of Ophthalmology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
  • Norbert Pfeiffer
    Department of Ophthalmology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
  • Joanna Wasielica-Poslednik
    Department of Ophthalmology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
  • Adrian Gericke
    Department of Ophthalmology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
  • Footnotes
    Commercial Relationships   Caroline Manicam, None; Natarajan Perumal, None; Yong Ngongkole, None; Alexandra Tschäbunin, None; Marcel Sievers, None; Franz Grus, None; Norbert Pfeiffer, None; Joanna Wasielica-Poslednik, None; Adrian Gericke, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 3060. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Caroline Manicam, Natarajan Perumal, Yong Cajetan Ngongkole, Alexandra Tschäbunin, Marcel Sievers, Franz H Grus, Norbert Pfeiffer, Joanna Wasielica-Poslednik, Adrian Gericke; The influence of hard and soft contact lenses on tear protein profiles: A perspective through the proteomic looking glass. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3060.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : The prevalence of contact lens (CL) use is increasing worldwide owing to various factors. Albeit the many benefits, the adverse effects of CL wear on the ocular surface are no less important. To date, many studies had assessed the pathophysiology of CL use on tear film, the main component of the anterior eye that is directly affected by CLs. However, in-depth studies at the protein level are still lacking. Hence, this study endeavoured to elucidate the differentially expressed protein profiles in tears of hard and soft CL users and, to investigate for the first time the proteome changes associated with renouncement of CL employing the mass-spectrometry (MS)-based proteomic platform.

Methods : Tear samples were collected from non-CL users (Controls) (N= 22), hard CL (N= 16) and soft CL (N= 18) users. Tears were collected before and after renouncement (for 4.7 ± 0.7 days) of CL use. Samples were pooled in each group and subjected to label-free quantitative MS analyses. The acquired MS spectra were analysed by MaxQuant computational proteomics platform, followed by functional annotation and pathways analyses.

Results : A total of 261 proteins were identified in the tear samples with less than 1 % false discovery rate. Among these, 93 proteins were significantly (P <0.01) differentially expressed in both CL groups. The differential expressions of some proteins were exclusive to a particular CL type, such as the HSPA8 and PRR4 in the hard CL and, ENO1 and PROL1 in the soft CL group. The top clusters of proteins significantly upregulated in both CL users were involved in inflammatory responses and metabolic diseases. Interestingly, many of these were restored to near-normal after renouncement, namely AGT, PIP, TF and MSLN. Conversely, specific protein clusters did not revert back to normal levels after renouncement especially FABP5, which was a common up-regulated protein in both groups.

Conclusions : In gist, this study elucidated the complex proteome alterations implicated in tears of both types of CL users. Importantly, these findings have provided first evidence that there are specific proteome changes attributed to renouncement of CL wear. These proteins might be the key players that maintain homeostasis and provide protection on the ocular surface.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×