June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Inhibition of HDAC6 suppresses epithelial–mesenchymal transition of lens epithelial cells through increasing α-tubulin acetylation
Author Affiliations & Notes
  • Shan Huang
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China
  • Liping Wang
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China
  • Xiaoxian Lu
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China
  • Baoxin Chen
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China
  • Jieping Chen
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China
  • Yizhi Liu
    State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China
  • Footnotes
    Commercial Relationships   Shan Huang, None; Liping Wang, None; Xiaoxian Lu, None; Baoxin Chen, None; Jieping Chen, None; Yizhi Liu, None
  • Footnotes
    Support   National Natural Science Foundation of China (No. 81300749)
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 3179. doi:
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      Shan Huang, Liping Wang, Xiaoxian Lu, Baoxin Chen, Jieping Chen, Yizhi Liu; Inhibition of HDAC6 suppresses epithelial–mesenchymal transition of lens epithelial cells through increasing α-tubulin acetylation. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3179.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Epithelial–mesenchymal transition (EMT) of lens epithelial cells are causally associated with anterior subcapsular cataract and posterior capsular opacification, which are critical causes of visual disorder. The objective of this work is to investigate the effect and mechanism of Histone deacetylase 6 (HDAC6), a tubulin-associated deacetylase, on epithelial–mesenchymal transition of lens epithelial cells.

Methods : The human lens epithelial cells (HLECs), SRA01/04, were transfected with small interfering RNAs (siRNA) of HDAC6 to inhibit HDAC6 expression, and treated with tubacin (5 μM), a deacetylase inhibitor, to suppress the deacetylation activity of HDAC6, respectively. After transfection of siRNA or treatment with tubacin, cells were cultured in the presence or absence of TGF-β2 (5 ng/mL) for 24-48 hours. The acetylated α-tubulin levels were detected by western blot and immunofluorescence staining. EMT markers, such as fibronectin (FN), collagen IV (Col IV) and α smooth muscle actin (α-SMA), were investigated at mRNA and protein levels by real-time PCR, western blot, as well as immunofluorescence staining analysis.

Results : The acetylated α-tubulin levels in HLECs were significantly increased after HDAC6 expression inhibition or deacetylase inactivation by tubacin treatment. Compared to the control group, both mRNA and protein expression levels of EMT markers (FN, Col IV and α-SMA) were decreased in HLECs of the HDAC6-siRNA transfected group, as well as the tubacin treated group.

Conclusions : Inhibition of HDAC6 expression could suppress the EMT of HLECs through promoting acetylation of α-tubulin, indicating that α-tubulin deacetylase might be a potential drug target for the prevention and treatment of anterior subcapsular cataract and posterior capsular opacification.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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