June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017

The Development of an Ocular Wound Chamber for the Treatment of Ocular Wounds and Periorbital Tissue
Author Affiliations & Notes
  • Gina L Griffith
    Ocular Trauma, United States Army Institute for Surgical Research, San Antonio, Texas, United States
  • Jennifer McDaniel
    Ocular Trauma, United States Army Institute for Surgical Research, San Antonio, Texas, United States
  • Elof Eriksson
    Harvard Medical School , Boston, Massachusetts, United States
  • Anthony Johnson
    Ocular Trauma, United States Army Institute for Surgical Research, San Antonio, Texas, United States
  • Footnotes
    Commercial Relationships   Gina Griffith, None; Jennifer McDaniel, None; Elof Eriksson, None; Anthony Johnson , None
  • Footnotes
    Support  This work was supported by the U.S. Army Medical Research and Materiel Command (MRMC) Clinical and Rehabilitative Medicine Research Program.
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 3377. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Gina L Griffith, Jennifer McDaniel, Elof Eriksson, Anthony Johnson;
      The Development of an Ocular Wound Chamber for the Treatment of Ocular Wounds and Periorbital Tissue. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3377.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : As therapies available to treat and heal ocular surface injuries and periocular burns are frequently inadequate, costly, and labor intensive, a flexible semi-transparent ocular wound chamber device has been developed to protect the ocular surface during periocular burn treatment, enhance the treatment of ocular infections, and promote the healing of periocular skin grafts. Our purpose is to test the hypothesis that this ocular wound chamber can be successfully and safely utilized in a guinea pig model to collect the pre-clinical data required to transition this technology.

Methods : Excess hair surrounding the left eye of anesthetized Institute Armand Frappier (IAF) hairless, female guinea pigs (Crl:HA-Hrhr) was removed using Nair™ (30 s). During hair removal, eyes were protected with Refresh® Lacri-Lube®. Post hair removal and cleaning of the skin with 70% EtOH, guinea pigs were fitted with the ocular wound chamber. Animals were randomly grouped (N=4 per group) and 0.5 ml of GenTeal® gel or balanced saline solution (BSS) was injected into the chamber. The left (OS) treatment eye and right (OD) control eye were assessed at 0, 24, and 72 h post chamber placement utilizing intraocular pressure (IOP) and pachymetry measurements as well as slit lamp, white light, fluorescein, and OCT imaging. Corneal and eyelid skin histology was performed using hematoxylin and eosin (H&E) staining. Histological sections were scored by a board certified pathologist.

Results : IOP measurements revealed an average IOP of 10 ± 2 mmHg. The average corneal thickness was 200 ± 25 μm. No significant differences were found between the IOP, corneal thickness, slit lamp imaging, fluorescein staining, or OCT imaging in either treatment group (GenTeal® or BSS) when compared to control eyes. Histological scores totals for the cornea (GenTeal®: 0.3, BSS: 0, Control: 0) and eyelids (GenTeal®: 3.0, BSS: 2.6, Control: 1.2) revealed no pathological differences between treatment groups and controls.

Conclusions : This biocompatible wound chamber can be safely and effectively used with GenTeal® or BSS in our guinea pig model without adverse effects on the ocular or periocular tissue. These results advance the overall efforts to develop this ocular wound chamber for the treatment of ocular surface injuries and periocular burns. Future studies aim to treat ocular injuries and infections while allowing the periorbital skin to heal.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×