June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Samd7, a photoreceptor-specific component of the polycomb repressive complex, plays an essential role in repressing non-rod genes expression through H3K27me3 regulation in rod photoreceptors
Author Affiliations & Notes
  • Takahisa Furukawa
    Molecular and Developmental Biology, Osaka Univ. Inst for Protein Rsrch, & JST, CREST, Suita, Japan
  • Shun Kubo
    Molecular and Developmental Biology, Osaka Univ. Inst for Protein Rsrch, & JST, CREST, Suita, Japan
  • Mayu Furuhashi
    Molecular and Developmental Biology, Osaka Univ. Inst for Protein Rsrch, & JST, CREST, Suita, Japan
  • Akiko Ueno
    Molecular and Developmental Biology, Osaka Univ. Inst for Protein Rsrch, & JST, CREST, Suita, Japan
  • Tetsuo Kon
    Molecular and Developmental Biology, Osaka Univ. Inst for Protein Rsrch, & JST, CREST, Suita, Japan
  • Taro Chaya
    Molecular and Developmental Biology, Osaka Univ. Inst for Protein Rsrch, & JST, CREST, Suita, Japan
  • Shinji Ueno
    Nagoya Univ School of Med, Nagoya, Japan
  • Yoshihiro Omori
    Molecular and Developmental Biology, Osaka Univ. Inst for Protein Rsrch, & JST, CREST, Suita, Japan
  • Footnotes
    Commercial Relationships   Takahisa Furukawa, None; Shun Kubo, None; Mayu Furuhashi, None; Akiko Ueno, None; Tetsuo Kon, None; Taro Chaya, None; Shinji Ueno, None; Yoshihiro Omori, None
  • Footnotes
    Support  Japan Science and Technology Agency, Grant-in-Aid for Scientific Research
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 3559. doi:
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      Takahisa Furukawa, Shun Kubo, Mayu Furuhashi, Akiko Ueno, Tetsuo Kon, Taro Chaya, Shinji Ueno, Yoshihiro Omori; Samd7, a photoreceptor-specific component of the polycomb repressive complex, plays an essential role in repressing non-rod genes expression through H3K27me3 regulation in rod photoreceptors. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3559.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Functions and molecular mechanisms of multiple transcription factors in rod and cone photoreceptor-specific gene regulation have been well studied. On the other hand, the functional mechanisms underlying the epigenetic regulation in photoreceptor cell type specification has been poorly understood.

Methods : In the current study, we analyzed biochemical and biological functions of Samd7, a rod photoreceptor-specific SAM domain protein.

Results : We observed that various non-rod genes including S-opsin are ectopically expressed in rod photoreceptor cells and multiple rod genes were significantly down-regulated in the Samd7-null retina. We found that Samd7 physically interacts with components of polycomb repressive complex 1 (PRC1), Polyhomeotic homologs 1-3 (Phc1-3). Chromatin immunoprecipitation assay revealed that multiple genes up-regulated in the Samd7-deficient retina exhibited a significant decrease of the H3K27me3 marks.

Conclusions : Our results suggest that Samd7 plays an essential role in repressing non-rod genes expression through H3K27me3 regulation in rod photoreceptors.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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