June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Tear Cytokine Profile in a Scandinavian Cohort of Congenital Aniridia
Author Affiliations & Notes
  • Erlend Christoffer Sommer Landsend
    Department of Ophthalmology, Oslo University Hospital, Oslo, Norway
  • Hilde Rogeberg Pedersen
    Faculty of Health Sciences, National Centre for Optics, Vision and Eye Care, University College of Southeast Norway, Kongsberg, Norway
  • Øygunn Utheim
    Department of Ophthalmology, Oslo University Hospital, Oslo, Norway
  • Hans Christian Aass
    Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway
  • Behzod Tashbayev
    The Norwegian Dry Eye Clinic, Oslo, Norway
  • Neil S Lagali
    Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden
  • Darlene A Dartt
    Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States
  • Rigmor C Baraas
    Faculty of Health Sciences, National Centre for Optics, Vision and Eye Care, University College of Southeast Norway, Kongsberg, Norway
  • Tor Utheim
    Faculty of Health Sciences, National Centre for Optics, Vision and Eye Care, University College of Southeast Norway, Kongsberg, Norway
    Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway
  • Footnotes
    Commercial Relationships   Erlend Landsend, None; Hilde Rogeberg Pedersen, None; Øygunn Utheim, None; Hans Christian Aass, None; Behzod Tashbayev, None; Neil Lagali, None; Darlene Dartt, None; Rigmor Baraas, None; Tor Utheim, None
  • Footnotes
    Support  The Norwegian Ophthalmological Society and Professor Hjalmar Schiötz Memorial Fund grants and grants for PhD in Strabismus/ Pediatric Ophthalmology. Inger Holm’s Memorial Fund grant
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 3572. doi:
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      Erlend Christoffer Sommer Landsend, Hilde Rogeberg Pedersen, Øygunn Utheim, Hans Christian Aass, Behzod Tashbayev, Neil S Lagali, Darlene A Dartt, Rigmor C Baraas, Tor Utheim; Tear Cytokine Profile in a Scandinavian Cohort of Congenital Aniridia. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3572.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To investigate the tear cytokine profile in a Scandinavian cohort of congenital aniridia in a case-control study, and to examine the correlation between cytokine levels and ophthalmological findings.

Methods : Thirty-five patients with congenital aniridia and 21 healthy controls were examined. Tear fluid was collected using Schirmer I test and capillary tubes, and the concentration of 27 human cytokines was determined in the fluid from both eyes separately employing a multiplex bead assay. All participants also underwent an extensive ophthalmological examination including systematic slit-lamp biomicroscopy, measurement of tear production and ocular surface staining, and evaluation of meibomian glands (meibography). Differences in cytokine levels between the two groups were analysed, and correlations between levels in the aniridia group and ophthalmological findings were calculated.

Results : Significantly elevated concentrations were shown for 16 cytokines in tear fluid from both right and left eyes of aniridia patients compared to the controls. These 16 were: interleukin 1ß (IL-1ß), IL-4, IL-6, IL-9, IL-12p70, IL-13, IL-15, IL-17A, eotaxin, basic fibroblast growth factor (basic FGF), granulocyte colony-stimulating factor (G-CSF), interferon gamma (IFN-γ), monocyte chemoattractant protein 1 (MCP-1), macrophage inflammatory protein 1α (MIP-1α), and MIP-1ß. Interestingly, IL-1ß and IL-6, which are suggested to play a role in dry eye disease (DED), demonstrated a substantial increase in concentration in the anridia group compared to the control group (IL-1ß: 20.3 pg/ml vs 0.7, p<0.001; IL-6: 6.5 pg/ml vs 1.7, p=0.001). The level of interferon gamma-induced protein 10 (IP-10) and regulated-on-activation, normal T cell expressed and secreted (RANTES) correlated significantly with stage of aniridic keratopathy (R=-0.451, p=0.018 and R=0.435, p=0.023, respectively).

Conclusions : Tear fluid of aniridia patients demonstrated significantly elevated levels of several cytokines related to inflammation compared to control individuals, and a moderate correlation between aniridic keratopathy and concentrations of IP-10 and RANTES was found. Increased inflammation of the ocular surface may be a contributory factor explaining previous reports of high prevalence of dry eye disease in aniridia patients.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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