June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Cold Shock Proteins Expression in the Retina Following Exposure to Hypothermia
Author Affiliations & Notes
  • Ignacio M Larrayoz
    Biomarkers and Molecular Signaling, Center for Biomedical Research of La Rioja (CIBIR), Fundación Rioja Salud, Logroño, La Rioja, Spain
  • Manuel Rey-Funes
    Neuropatología Experimental, Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), Facultad de Medicina, UBA , Buenos Aires, Argentina
  • Daniela S Contartese
    Neuropatología Experimental, Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), Facultad de Medicina, UBA , Buenos Aires, Argentina
  • Federico Rolón
    Neuropatología Experimental, Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), Facultad de Medicina, UBA , Buenos Aires, Argentina
  • Aníbal Sarotto
    Neuropatología Experimental, Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), Facultad de Medicina, UBA , Buenos Aires, Argentina
  • Verónica B Dorfman
    Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y Diagnóstico (CEBBAD), Universidad Maimónides, Buenos Aires, Argentina
  • César F Loidl
    Neuropatología Experimental, Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), Facultad de Medicina, UBA , Buenos Aires, Argentina
  • Alfredo Martinez
    Angiogenesis, CIBIR, Fundación Rioja Salud, Logroño, Spain
  • Footnotes
    Commercial Relationships   Ignacio Larrayoz, None; Manuel Rey-Funes, None; Daniela Contartese, None; Federico Rolón, None; Aníbal Sarotto, None; Verónica Dorfman, None; César Loidl, None; Alfredo Martinez, None
  • Footnotes
    Support  Instituto Carlos III -Miguel Servet Program CP15/00198 (Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 de concesión de la de ayuda de contratos Miguel Servet, por el Fondo Social Europeo (Periodo de Programación 2014 – 2020))to IML and US Department of Defense, Grant MR130239 (to AM)
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 3592. doi:
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    • Get Citation

      Ignacio M Larrayoz, Manuel Rey-Funes, Daniela S Contartese, Federico Rolón, Aníbal Sarotto, Verónica B Dorfman, César F Loidl, Alfredo Martinez; Cold Shock Proteins Expression in the Retina Following Exposure to Hypothermia. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3592.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Hypothermia has been proposed as a therapeutic intervention for some retinal conditions, including ischemic insults. Cold exposure elevates expression of cold-shock proteins (CSP), including RNA-binding motif protein 3 (RBM3) and cold inducible RNA-binding protein (CIRP), but their presence in mammalian retina is so far unknown

Methods : Two cell lines of retinal origin, R28 and mRPE, were exposed to 32°C for different time periods and CSP expression was measured by qRT-PCR and Western blotting. Neonatal and adult Sprague-Dawley rats were exposed to a cold environment (8°C) and expression of CSPs in their retinas was studied by Western blotting, multiple inmunofluorescence, and confocal microscopy

Results : RBM3 expression was upregulated by cold in both R28 and mRPE cells in a time-dependent fashion. On the other hand, CIRP was upregulated in R28 cells but not in mRPE. In vivo, expression of CSPs was negligible in the retina of newborn and adult rats kept at room temperature (24°C). Exposure to a cold environment elicited a strong expression of both proteins, especially in retinal pigment epithelium cells, photoreceptors, bipolar, amacrine and horizontal cells, Müller cells, and ganglion cells

Conclusions : In conclusion, CSP expression rapidly rises in the mammalian retina following exposure to hypothermia in a cell type-specific pattern. This observation may be at the basis of the molecular mechanism by which hypothermia exerts its therapeutic effects in the retina

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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