June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Evaluation of conjunctival and lacrimal gland inflammation after corneal herpes simplex virus-1 infection
Author Affiliations & Notes
  • Pushpa Rao
    Anatomy and Cell Biology, Wayne State University School of Medicine, Detriot, Michigan, United States
  • Subhash Gaddipati
    Ophthalmology , Wayne state university School of medicine, Detriot, Michigan, United States
    Anatomy and Cell Biology, Wayne State University School of Medicine, Detriot, Michigan, United States
  • Bala Burugula
    Ophthalmology , Wayne state university School of medicine, Detriot, Michigan, United States
    Anatomy and Cell Biology, Wayne State University School of Medicine, Detriot, Michigan, United States
  • Andrew Jerome
    Anatomy and Cell Biology, Wayne State University School of Medicine, Detriot, Michigan, United States
  • Robert L McKown
    Department of Integrated Science and Technology , James Madison University , Harrisonburg, Virginia, United States
  • Gordon W Laurie
    Department of Cell Biology and Ophthalmology, University of Virginia , Charlottesville, Virginia, United States
  • Susmit Suvas
    Ophthalmology , Wayne state university School of medicine, Detriot, Michigan, United States
    Anatomy and Cell Biology, Wayne State University School of Medicine, Detriot, Michigan, United States
  • Footnotes
    Commercial Relationships   Pushpa Rao, None; Subhash Gaddipati, None; Bala Burugula, None; Andrew Jerome, None; Robert McKown, None; Gordon Laurie, None; Susmit Suvas, None
  • Footnotes
    Support  NH Grant EY022417, NH Grant EY004068, Research to Prevent blindness
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 3620. doi:
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      Pushpa Rao, Subhash Gaddipati, Bala Burugula, Andrew Jerome, Robert L McKown, Gordon W Laurie, Susmit Suvas; Evaluation of conjunctival and lacrimal gland inflammation after corneal herpes simplex virus-1 infection. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3620.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Tear production is controlled by corneal innervation, main lacrimal gland and the lacrimal functional unit. Recent studies from our lab showed a significant reduction in tear volume in HSV-1 infected corneas of C57BL/6J (B6) mice during clinical disease period. Reduced tear volume was associated with hypoxia marker, pimonidazole, staining in the corneal epithelium of HSV-1 infected eyes. In this study, we ascertained whether corneal HSV-1 infection causes conjunctival and lacrimal gland inflammation in a B6 mouse model. Moreover, the studies are carried out to determine if increasing the tear volume in HSV-1 infected eyes prevents the development of corneal hypoxia in infected eyes.

Methods : Mild corneal scratching was carried out prior to the topical application of infectious virus. Extra-orbital lacrimal gland (EOLG) and conjunctival tissue was excised from uninfected and HSV-1 infected B6 mice at different days post-infection (p.i.). Enzymatic digestion of the excised tissues was carried out to obtain a single cell suspension for flow cytometry. To increase tear volume in HSV-1 infected eyes, topical application of lacritin was carried out 4x/ day from day 4 through 10 p.i. Control group of infected mice received C-25 (a lacritin truncation mutant). Phenol red thread test was carried out to measure the tear volume. Corneal smoothness of infected eyes was evaluated using reflected eye images from the LED ring illuminator of a stereomicroscope.

Results : A massive influx of leukocytes involving CD4 and CD8 T cells was noted in inflamed conjunctival and EOLG tissues on day 10 p.i. Lacritin, but not C-25, treatment effectively delays the decrease in tear volume in infected B6 mice. However, the lacritin treatment alone was not effective in preventing the loss of corneal epithelium smoothness seen in HSV-1 infected eyes during clinical disease period. Moreover, lacritin treatment did not decrease leukocyte influx in EOLG and pimonidazole staining in corneas of infected B6 mice.

Conclusions : Together, our results suggest that leukocyte influx in EOLG and conjunctival tissue may play an important role in reducing tear volume after ocular HSV-1 infection. Although lacritin treatment alone delays the decrease in tear volume in infected eyes, it was not sufficient to inhibit leukocytes influx in EOLG and the corneal epithelial changes.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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